NO与GSH-PX在HIE的含量测定及临床意义

Testing concentrations of NO,GSH-PX of newborn with HIE

目的探讨新生儿缺氧缺血性脑病(HIE)血浆和脑脊液(CSF)中的一氧化氮(NO)和谷胱甘肽过氧化物酶(GSH-PX)含量变化及其与HIE不同时期和不同程度间相关关系。方法对HIE患儿第3天的CSF和出生初入院(HIE2h内)、第1天、第2天、恢复期的血浆中ND、GSH-PX进行检测,并与正常对照组比较;分析NO、GSH-PX的变化原因和意义。结果HIE患儿血浆中第1天的NO含量最高,而GSH-PX相反;初入院、第1天、第3天的血浆中NO、GSH-PX含量与正常对照组对比差异均有显著性(P<0.05),血浆和HIE第3天的CSF中和GSH-PX水平均呈负相关;病情越重NO浓度越高,GSH-PX越低。结论NO、GSH-PX均参与HIE的发病过程,在HIE的发病过程中起着重要作用;检测血浆和CSF中NO、GSH-PX含量有助于判断HIE患儿病变程度和病情进展。

[Objection] To stray the exprassion of the nitnic oxide(NO), Glutathion Peroxidase (GSH-PX) in plasma and cerebrospinal fluid (CSF) of neonate bypoxic-ischemic encephalopathy (HIE) and the retations among their expressions in different periods and degrees of HIE. [Methods] Tested the concentratiorrs of NO and GSH-PX in CSF of newborn infants suffered HIE 3 days and in plasma of newborn jnfants surrered HIE just attacked instant (HIE within 2 hour), 1 day, 3 days and restoring stage and Compared with them of normal contrast term. Analysed the reasons and sig nificance of NO, GSH-PX changes. [Results] The NO concentration was the highest in plasnra of nesborn infants suffered HIE 1 day. The GSH-PX concentrations in plasmas of newborn jnfants suffered HIE jusu attacked instant, 1day, 3days with of normal contrast team respectively. But there isn't significante difference (P >0.05) between them in plasmas of HIE on restoring stage and of normal contrast team. It was negative correlation whatever between the NO concentration and the GSH-PX concentration in plasma or in CSF of newborn infants suffered HIE 3 days. The more serious the disease was, the higher the NO concentration was, the lower the GSH-PX concentrations was. [Conclusion] NO,GSH-PX Play along with the course of HIE and a important role in neonate HIE. Testing the concentration of NO, and GSH-PX in plasma and in CSF can also help to judge the degree of disease and the cours of disease.