对HBsAg和抗-HCV ELISA筛查阳性献血者进行确认试验分析

Analysis of confirmatory test on blood donors for HBsAg and anti-HCV positive by ELISA

目的探讨对HBsAg和抗-HCVELISA筛查阳性献血者进行确认试验分析的意义。方法分别采用中和试验对ELISA检测HBsAg阳性献血者和重组免疫印记试验(RIBA)对抗-HCV阳性献血者进行确认检测,并对确认试验阴性献血者进行NAT检测。结果38693份献血者中,ELISA筛查出HBsAg和抗-HCV阳性分别为381(0.98%)份和173(0.45%)份。在ELISA筛查出的381份HBsAg阳性献血者中,经中和试验确认HBsAg阳性352份,29份为阴性;173份抗-HCV阳性献血者中,RIBA试验确认79份阳性,59份阴性,35份为可疑;29份中和试验确认HBsAg阴性标本和94份RIBA确认抗-HCV阴性或可疑的标本,NAT检测HBVDNA和HCVRNA均为阴性。结论对献血者进行HBsAg和抗-HCV确认试验能够避免假阳性结果的发生,有利于保护献血者的利益和推动志愿无偿献血事业的开展。

[0bjective] To evaluate the importance of confirmatory test for detecting HBsAg and anti-HCV in blood donors. [Methods] The samples of HBsAg positive by ELISA were confirmed by neutralization test and the samples of anti-HCV positive were confirmed by RIBA 3.0. The negative samples of confirmatory test were further detected by nucleic acid amplification test (NAT). [Results] Among 38693 samples from blood donors, 381(0.98%)samples were HBsAg positive and 173(0.45%)samples were anti-HCV positive by ELISA. Of 381 HBsAg positive samples by ELISA, 352 samples were positive for HBsAg and 29 samples were negative by neutralization test. Of 173 anti-HCV positive samples by ELISA, 79 samples were positive for anti-HCV, 35 samples were and 59 samples were negative by RIBA. 29 HBsAg-negative and 94 anti-HCV- indeterminate or negative samples by confirmatory test was HBV-DNA negative or HCV-RNA negative by NAT. [Conclusion] The result shows that HBsAg and anti-HCV false-positive samples should be avoided by confirmatory test. It can protect blood donors and impel voluntary nonremunerated donation.