抑癌基因FHIT和PTEN在肝癌中的表达及意义

Expression of FHIT and PTEN in primary hepatocellular carcinoma

目的探讨脆性组氨酸三联体(FHIT)基因和PTEN基因在原发性肝细胞癌(简称肝癌)中的表达及其与临床病理因素的关系。方法采用免疫组织化学SP法和图像分析技术检测了46例肝癌组织及癌旁组织和10例正常肝组织中FHIT和PTEN基因蛋白的表达。结果46例肝癌组织中FHIT和PTEN蛋白免疫组化阳性反应颗粒的平均光密度分别为(0.1339±0.3864)、(0.1534±0.1562),均明显低于相应癌旁组织(0.2051±0.5372)、(0.3271±0.2371)和正常组织(0.2289±0.7589)、(0.3753±0.0519)的水平(P<0.05);FHIT、PTEN与肝癌的病理分级、合并门静脉癌栓有密切关系。结论FHIT和PTEN基因蛋白缺失在肝癌组织中是频发事件。FHIT和PTEN与肝癌的病理分级、合并门静脉癌栓有密切关系。

Objective To investigate the expression of fragile histidine triad(FHIT) and PTEN in primary hepatocellular carcinoma(HCC) and their relationship to clinicopathological factors.Methods Immunohistochemical(S-P) and image analysis methods were used to detect the expression of FHIT and PTEN in the 46 patients with HCC and 10 normal liver tissues.Results (1) In the patients with HCC,average optical density of FHIT and PTEN in the tumor tissue was(0.1339±1.3864) and( 0.1534±0.1562) respectively,which was significantly lower than that in adjacent non-tumorous tissue and normal tissue(P<0.05).(2) The expression of both FHIT and PTEN was related to the tumor differention and thrombus in the portal vein.Conclusion (1) The lower expression of FHIT and PTEN gene protein is a frequent event in HCC.(2) The relationship between the expression of FHIT,PTEN and tumor parameters is obvious.