CK34_βE12、p63和p504S联合检测在前列腺肿瘤鉴别诊断中的应用

Application of CK34_βE12, p63 and p504S in diagnosis of prostate cancer

目的 探讨CK34βE12、p6 3和p5 0 4S联合免疫组化染色在前列腺良、恶性病变鉴别诊断中的价值。 方法 采用免疫组化方法 ,观察CK34βE12、p6 3、p5 0 4S在不同前列腺疾病中的表达情况。 结果 大多数良性前列腺增生及低度PIN的腺泡和导管周围可见连续的CK34βE12和p6 3(+) ,少数呈间断表达 ,尤以CK34βE12染色明显 ,但p5 0 4S染色皆呈 (- ) ;高度PINCK34βE12、p6 3染色呈不连续表达或 (- ) ,而增生的腺上皮 ,部分细胞p5 0 4S呈弱 (+)或(+) ;非典型腺瘤性增生 ,CK34βE12、p6 3呈 (+) ,p5 0 4S呈阴性反应 ;前列腺癌 2 0例中 19例CK34βE12、p6 3染色呈(- ) ,1例显示局灶性 (+) ,但p5 0 4S均呈 (+)。结论 p6 3、p5 0

Objective To evaluate the value of CK34βE12, p63, p504S immunostaining in diagnosis of benign and malignant lesions of the prostate. Methods Expression of CK34βE12, p63 and p504S in benign and malignant lesions of prostate was observed by immunohistochemical method. Results Continuous positive expression of CK34βE12 and p63 was found in the acini and ducts of most benign prostatic hyperplasia and low prostatic intraepithelial neoplasia (PIN), and few cases showed interrupted expression, especially by CK34βE12 staining, but p504S staining presented negative reaction; Interupted expression or negative reaction was found in high PIN cases and a part of hyperplastic glandular epithelia presented weekly positive or positive for p504S; Atypical aolenomatous hyperplasia was positive for CK34βE12 and p63, but negative for p504S; CK34βE12 and p63 staining was negative in 19 out of 20 prostatic carcinoma cases, 1 case presented focally positive reaction by CK34βE12 and p63, but negative by p504S. Conclusion Combined detection of p63 and p504S can be most helpful for diagnosis of prostatic carcinoma.