摘要
采用LichrospherC18柱,以甲醇和水为流动相,流速0.3mL/min,甲醇从60%～100%线性梯度洗脱25min,液质联用,电喷雾(ESI),对己烷雌酚及其衍生物己烷雌酚单(双)丁酸乙酯基醚分别进行了分离和结构鉴定。以混合酸酐法将己烷雌酚单羧基丙基醚(HESMCPE)与载体蛋白偶联,形成完全抗原,并以紫外扫描分析完全抗原的偶联率。经计算,HESMCPEBSA偶联率为36。
Using liquid chromatography /spectrometry (ESI), phase B (methyl alcohol), phase A (water), 0.3 mL/min of flow rate, a linear gradient from 60% to 100% of phase B and eluate for 25 min on a Lichrospher C-18 column, hexoestrol and it’s derivates hexoestrol-mono-ether-butyrate-ethyl (HES-MEBE), hexoestrol-di-ether-butyrate-ethyl (HES-DEBE) were separated and identified. The conjugates of hexoestrol-mono-caroxyl-propyl-ethyl (HES-MCPE) with proteins as synthetic antigens were prepared by the mixed anhydride method and the conjugation rate was analyzed by UV scanning. The conjugation rate is 36.
出处
《中国兽医科技》
CSCD
北大核心
2005年第7期561-564,共4页
Chinese Journal of Veterinary Science and Technology
基金
国家自然科学基金资助项目(20475022)
