酒石酸锑钾在诱导人肝癌BEL-7402细胞凋亡中的影响

Effect of Apoptosis by Potassium Antimonyl Tartrate in Human Hepatoma Cell Line in Vitro

目的:本研究旨在明确酒石酸锑钾(PAT)在体外对人肝癌BEL7402细胞凋亡的影响及抑癌机制。方法:用PAT以不同浓度、不同时间作用于人肝癌BEL7402细胞,以诱导其凋亡。用MTT比色法观察其细胞毒性,荧光显微镜、透射电镜、TUNEL染色法及流式细胞术(FCM)等方法来检测凋亡,观察其形态学和生化方面的变化。结果:PAT以剂量依赖和时间依赖的方式抑制BEL7402细胞的生长。5～40μmol·L-1的PAT处理48h后,形态学上,肝癌细胞表现为细胞皱缩、核质浓缩、核碎裂、细胞起泡以及凋亡小体形式等凋亡特征的形态学改变。DNA末端原位标记染色法、流式细胞仪均能检测到凋亡细胞。结论:PAT在体外诱导肝癌BEL7402细胞凋亡,能作为一种凋亡诱导剂用于肝癌的治疗。

Objective: To investigate the effects of apoptosis induced by potassium antimonyl tartrate (PAT) in human hepatoma cell line BEL-7402 in vitro. Methods: The selected human hepatoma cell line BEL-7402 was treated with PAT at various concentrations and for different time. Growth suppression and apoptosis-related alterations were evaluated by MTT method, Hoechst 33 258, transmission electron microscopy, the TDT-mediated dUTP nick end-labeling assay(TUNEL) and flow cytometry (FCM). Results: PAT inhibited the growth of hepatoma cells in a dose-and time-dependent manner. After the treatment with 5~40 μmol·L^(-1) PAT for 48 hours, apoptosis with nuclear chromatin condensation and fragmentation as well as cell shrinkage and the formation of apoptotic bodies were observed using fluorescent microscope and transmission electron microscopy. Apoptotic cells were also detected by flow cytometry and TUNEL staining. Conclusion: PAT induces apoptosis of human hepatoma cell line BEL-7402 and may be a promising apoptosis-inducer in hepatoma therapy.