摘要
目的:观察骨折愈合过程中骨痂组织内骨形态发生蛋白-2(BMP-2)和血管内皮细胞生长因子(VEGF)的表达及分布情况,并探讨其作用机制。方法:以Wistar大鼠为研究对象,制作股骨骨折愈合模型,采用免疫组织化学和HE染色的方法,检测骨折不同阶段(1,3,7,14,21,28,42d)BMP-2、VEGF的表达、变化和分布。结果:在骨折愈合的不同阶段,骨痂组织内表达BMP-2、VEGF的细胞来源不同,同时表达程度也存在差异。结论:骨折愈合的不同时期,BMP-2、VEGF有着各自的表达和分布特点,并共同调节骨祖细胞的增殖和成骨细胞、软骨细胞的分化,最终完成骨折修复。
Objective: To observe the expression and distribution of bone morphogenetic protein-2 (BMP-2) and vascular endothelial growth factor (VEGF) during fracture healing. Methods: The models of femoral fracture healing of Wistar rat were established, and then the expression, variation and distribution of BMP-2 and VEGF in different healing stages of fracture (day 1, 3, 7, 14, 21,28, 42) were detected using immunohistochemical observation and histological observation. Results: In different stages of fracture healing, BMP-2 and VEGF were expressed in different cells of callus, and the degree of expression also varied. Conclusion: In different stages of fracture healing, BMP-2 and VEGF each has its own expression and distribution characteristics, and they cooperated in regulating the multiplication of osteoprogenitor cell and the differentiation of chondrocytes and osteoblasts for fracture healing.
出处
《武汉大学学报(医学版)》
CAS
2005年第4期467-469,i002,共4页
Medical Journal of Wuhan University
关键词
骨折愈合
骨形态发生蛋白
血管内皮细胞生长因子
Fracture Healing
Bone Morphogenetic Protein
Vascular Endothelial Growth Factor