Measurement of serum granulocyte colonystimulating factor levels in patients with different phase of infection

Objective: To detect the serum granulocyte colony-stimulating factor (G-CSF) levels between the patients with frequently repeated infection (repeaters) and others (non-repeaters) in different phase of infection. Methods: An enzyme-linked immunosorbent assay (ELISA) method was used to detect serum G-CSF levels in 50 cases (32 non-repeaters and 18 repeaters) with acute phase of infection. Serum G-CSF levels were detected in recovery phase in 10 cases. Results: Serum G-CSF levels were significantly higher (1429.97±506.43ng/L) in 32 non-repeaters with acute infection. There was a positive correlation between white blood cell count (WBC) and serum G-CSF level (r =0. 396, P <0.05). There was also a positive correlation between absolute neutrophil count (ANC) and serum G-CSF level (r=0.346,P<0,05). Serum G-CSF levels were higher (98.62 -+56.40ng/L) in 18 repeaters with acute infection, It was showed that serum G-CSF levels were significantly higher in non-repeaters than in repeaters with acute phase of infection ( P <0. 001 ). In the meanwhile, the body temperature was significantly higher in non-repeaters than in repeaters with acute infection (37.95±0.14℃ rs 36.91±0.13℃ ,P <0.001), There were no significant differences in age, WBC, ANC, type of bacterial, liver function and renal function (P > 0.05). Serum G-CSF levels in recovery phase of the two groups were below the sensitivity of the assay ( < 60 ng/L), Conlusion: It is suggested that application of recombinant G-CSF may be useful for the patients with repeated infection,