大额牛冷冻精液技术的研究

Study on Semen Freezing Method ofBos Frontalis

本文对大额牛的射精量、精子密度、精子活力等指标进行了测定,同时对大额牛精液的冷冻稀释液、制作程序进行了较为系统的研究。结果得出,大额牛在人工饲养条件下,公牛的射精量为4.92±1.50mL、精子密度为16.79±4.5亿/mL、原精活力0.63±0.078;冷冻精液解冻活力平均可达0.3875±0.0426、冻精解冻后顶体完整率平均52.74%±5.88%,畸形率为12.95%±3.18%,精子复苏率为59.63%±3.67%,其他指标与一般黄牛基本一致。

The volume of ejaculate, density and motility of spermatozoa ofBos frontaliswere measured, and the se- men dilution and freezing procedures were systematically examined. The results indicated that under the artificial feeding condition, the volume of ejaculate, the density and motility of spermatozoa were 4.92±1.50 mL, 16.79±45.0 million/ mL, 0.63±0.078, respectively, the average thawing motility of frozen semen was 0.388±0.024, percentage of intact acrosome of frozen semen was 52.74%±3.88%,percentage of abnormal sperm was 12.59%±2.14% and sperm sur- vival rate after thawing was 59.93%±3.67%, other indicators of reproductivity ofBosfrontaliswere similar toBos tau- rus.