五苓散及其组分对正常小鼠血浆心钠素含量的影响

Effect of Wu Lin Powder and Its Ingredients on Atrial Natriuretic Factor Level in Mice

五苓散作为利水化湿方已长期应用于临床，为了探明其利尿作用的机理，本研究观察了五苓散对小鼠血浆心钠素（ＡＮＦ）的影响。结果发现小鼠在五苓散灌胃４５ｍｉｎ后ＡＮＦ从５．４２±０．９６ｎｇ／ｍｌ升至８．８５±１．５４ｎｇ／ｍｌ（Ｐ＜０．０５），其方中泽泻（８．５７±１．９８ｎｇ／ｍｌ，Ｐ＜０．０５），桂枝（８．９３±１．４７ｎｇ／ｍｌ，Ｐ＜０．０１）也有明显升高ＡＮＦ的作用，且它们的作用较生理盐水明显，而ＡＮＦ具有明显的排钠利尿作用，所以推测ＡＮＦ是五苓散利尿作用的物质基础。

Wu Lin powder(WLP)is a prescription that causes urination to remove dampness. In order to elucidate its mechanism of action,the effect of WLP on atrial natriuretic factor(ANF)was observed.The results showed that the level of ANF was significantly higher in the plasma of mice after giving WLP as well as giving Alisma orientalis or Cinnamomum cassia orally,compared with control,P<0.05,0.01 and 0.001 respectively.The effect was most evident at 45 minute after giving WLP(P<0.05).It suggested that ANF might play an important role in therapeutic action of WLP.