维生素B_6营养状况对喂予硒酸钠大鼠组织中硒存留率的影响

Effect of vitamin B_6 status on selenium retention in the tissues in rats fed selenium from sodium selenate

目的研究维生素B6营养状况对组织中硒存留率的影响。方法选择4周龄初断乳雄性大鼠饲缺维生素B6、缺硒的酪蛋白蔗糖基础饲料,3周后按体重随机把动物分成6组,即在缺硒缺维生素B6基础饲料中补硒酸钠形式硒0.25mgkg和不同剂量的盐酸吡哆醇0、1.0、2.5和5.0mgkg。实验为期4周,在试验结束前3天,进行3天代谢试验,收集粪便。结果与补充维生素B6各组相比,缺乏维生素B6组的大鼠体重和饲料利用率显著降低,心、肝、肾脏重量和硒存留率也显著降低,但对睾丸重量和硒存留率没有显著影响;维生素B6的营养状况对硒的表观吸收率和存留率没有明显的影响。结论补充适宜量维生素B6有助于改善动物的生长发育和增加组织中硒存留。

Objective\ To study the effect of vitamin B_6 status on selenium retention in tissues. Methods Weaning male Wistar, 4-week-old, fed with vitamin B_6-selenium-deficient basal diet for 3 weeks was randomly divided into 6 groups. There were six experimental diets,that is basal diet ~vitamin B_6-selenium-deficient), basal diet supplemented with vitamin B_6, and supplemented with Se 0.25mg/kg as Na_2SeO_4 and vitamin B_6 0, 1.0 2.5 and 5.0mg/kg diet as pyridoxine HCl for 4 weeks, three-days metabolic test was carried out before finished the study. Results Compared with each group supplemented with vitamin B_6, the vitamin B_6 deficient groups had a significantly low body weight gain and diet availability, and lower selenium retention in heart, liver, and kidneys, however, the status of vitamin B_6 had no significant effect on the weight and selenium retention in the testes. Vitamin B_6 had no significant influence on the selenium apparent absorption and retention by the three days metabolic test. Conclusion Providing adequate vitamin B_6 could be benefit for improving rat growth and development and increasing selenium retention in the tissues.