N^+注入选育黑曲霉益生菌及其突变菌株产酶条件的研究

Study on breeding of probiotic Aspergillus nigre by N^+ implantation and fermentation condition of its mutant

以益生菌株黑曲霉AN01为材料,经N+多次诱变得突变益生菌株AN03。结果表明,出发益生菌株AN01酸性蛋白酶、纤维素酶和果胶酶的酶活分别由原来的71.6Ug、141.7Ug和264.8Ug相继提高到996.5Ug、940.4Ug和906.5Ug。突变益生菌株AN03经传5代培养,产酶特性稳定。试验还研究了变突变益生菌株AN03最佳产酶条件,培养基为每升含麸皮105g,玉米芯105g,豆粕105g,氯化铵16g,pH5.0。30℃培养4d。

Using Aspergillus niger AN01 as original strain, mutated strain Aspergillus nigerAN03 was obtained by N + implantation. The results showed that activities of acidic protease, cellulase and pectinase of Aspergillus niger were raised from 71.6U/g, 141.7U/g and 264.8U/g to 996.5U/g, 940.4U/g and 906.5U/g respectively. Characteristics of enzymes production in the mutant Aspergillus niger AN03 kept stabile by 5 times subculture.In addition, the optimum conditions for enzymes production of Aspergillus niger AN03 were investigated. The optimum components of medium consisted of bran 105.0g, corn straw 105.0g, bean cake 105.0g, NH-4Cl 6.0g, H-2O 1000mL.The optimum fermentation condition was incubated for 4d in the condition of pH 5.0 and temperature 30℃.