Fas/FasL的表达与星型细胞瘤分化关系的探讨

Study on the relationship between the expression of Fas/FasL gene and the differentiation of astrocytoma.

目的探讨Fas/FasL在人脑星形细胞瘤中的作用与意义.方法应用免疫组化法(S-P法)检测Fas和FasL在不同病理分级的人脑星形细胞瘤中的表达.结果 Fas和FasL在41例星形细胞瘤中阳性细胞的表达率分别为68.3%(28/41)和75.6%(31/41).Fas和FasL的表达水平在Ⅰ～Ⅱ级星形细胞瘤中表达为-或+的分别占95.0%和75.0%,而Ⅲ～Ⅳ级星型细胞瘤中表达为++和+++的分别占71.4%和66.7%.切片观察发现Fas和FasL表达阳性区集中多在缺血坏死灶周围,分别占67.8%和67.7%.Fas/FasL比值随着肿瘤的分级增高而更接近于1.0.结论正常脑组织不表达Fas和FasL,在星形细胞瘤中可见Fas和FasL的表达.在星形细胞瘤中Fax/FasL的表达情况可反映神经胶质细胞间变程度及星形细胞瘤恶性变时瘤细胞增殖的程度.

Objective To study the relationship between Fas expression and the differentiation of astrocytoma. Methods The expression intensity of the Fas and FasL in different pathology grade of astrocytoma has been determined by using immunohistochemistry (S-P method).There were totally 50 examples, including 41 astrocytoma examples. Results The expressing rate of positive cell is 68.3%(28/41)and 75.6%(31/41)respectively in 41 case astrocytoma. the expression of Fas/FasL in astrocytoma was certainly associated with their pathology grade. In 41 case, the expression of Fas/fasL which is - or + in 1～2 grades astrocytoma occounted for 95.0% and 75.0% respectively, and occounted for 71.4% and 66.7% respectively in 3～4 grades of astrocytoma. The positive area of Fas/FasL expression concentrated around ischemia and necrosis area after the pathology section in 41 example patients, and occounted for 92.9% and 96.8% respectively. Conclusion Normal brain tissue does not express Fas/FasL, but the expressing of Fas/FasL can be found in astrocytoma. The expressing of Fas/FasL can reflect the anaplastic level of glioma and the tumour cell doubling extent when astrocytoma becomes malignant.