大黄素及番泻甙和大黄多糖对培养大鼠肝细胞内游离钙浓度的影响

Effects of Emodin, Sennosides and Rheum Polysaccharides on Free Calcium in Isolated Rat Liver Cells

应用钙荧光指示剂乙酰羟甲酯研究大黄素（ＥＭＤ）、番泻甙（ＳＥＮ）和大黄多糖（ＲＰＰ）对大鼠肝细胞内游离钙（［Ｃａ２＋］ｉ）浓度的影响。结果表明，在静息状态下肝细胞内［Ｃａ２＋］ｉ为１３１．６０±３７．７９ｎｍｏｌ／Ｌ。依次加入ＣａＣｌ２（２ｍｍｏｌ／Ｌ）和ＫＣｌ（１２０ｍｍｏｌ／Ｌ）后，肝细胞内［Ｃａ２＋］ｉ较静息时显著增加（Ｐ＜０．０１）。预先经ＥＭＤ（０．０３７ｍｍｏｌ／Ｌ）作用的肝细胞，在静息状态或加入同上剂量的ＣａＣｌ２和ＫＣｌ后，细胞内［Ｃａ２＋］ｉ均较去离子水对照组明显增加（Ｐ＜０．０１）；相反，ＳＥＮ和ＲＰＰ使肝细胞内［Ｃａ２＋］ｉ明显降低（Ｐ＜０．０１），且抑制效应与剂量呈负相关。从大黄不同有效成分ＥＭＤ、ＳＥＮ和ＲＰＰ对细胞内游离钙水平的不同影响提示，大黄对肝细胞功能具有多种调节作用。

The effects of emodin(EMD),sennosides(SEN) and Rheum polysaccharides(RPP) on the free calcium level in the isolated rat liver cells were studied with Ca2+ level 131.60±37.79nmol/L in the liver cells.After adding CaCl2(2mmol/L) and KCl(120mmol/L) to liver cell suspension sequentially,the free Ca2+ levels were significantly elevated compared with that of the resting status(P<0.01).When the liver cells were pretreated with EMD(0.037mmol/L) for 10 min, in the resting status or using the above doses of CaCl2 and KCl,the free Ca2+ levels were significantly increased compared with that of the control groups(P<0.01).On the contrary,after administration of SEN(0.046～0.092mmol/L) and RPP(0.1～0.2 mg/ml),the free Ca2+ levels were obviously decreased compared with that of the control groups(P<0.01).Furthermore,the inhibitory effect was dose dependent.The opposite effects of the different active ingredients of rhubarb(EMD,SEN,RPP) on the free Ca2+ levels suggested that rhubarb has many kinds of regulatory function in the liver cells.