子宫肌瘤患者外周静脉与子宫静脉血管内皮细胞生长因子水平测定及临床意义

VEGF Measurement in Uterine Vein and Peripheral Vein of Hysteromyoma Patients

目的:探讨血管内皮细胞生长因子(VEGF)在子宫肌瘤发生和肌瘤生长中的作用。方法:手术中暴露子宫静脉后,抽取子宫静脉血3ml,同时抽取非输液肢体外周静脉血3ml。抽取配对正常妇女外周静脉血3ml。ELISA法测定VEGF水平。测量子宫重量、肌瘤总重量、肌瘤数目和最大肌瘤最大径线值。结果:子宫肌瘤患者子宫静脉血和外周静脉血VEGF水平分别为159.38±25.63pg/ml和76.35±26.61pg/ml,对照人群外周静脉血VEGF水平为42.53±29.47pg/ml。子宫静脉血中的VEGF水平明显高于外周静脉血(P=0.019),子宫肌瘤患者外周静脉血中的VEGF水平明显高于对照人群外周静脉血的VEGF水平(P=0.013)。子宫重量、肌瘤总重量、肌瘤数目、最大肌瘤最大径线都与子宫肌瘤患者子宫静脉和外周静脉VEGF呈显著正相关。结论:VEGF可能参与了子宫肌瘤的生长和病情发展。

Objective: To investigate the serum vascular endothelial growth factor (VEGF) level of uterine vein and peripheral vein in uteromyoma patients and to test the hypothesis that VEGF are responsible for the myoma growth.Methods: 3ml blood sample of uterine vein and peripheral vein of hysteromyoma patients during uterine vein exposure underwent hysterectomy and 3ml peripheral vein of hysteromyoma patients and normal controls were collected, the method of serum VEGF measurement was ELISA. The uterine weight, total weight of myomas, the number of myomas and the maxium diameter of the lagest myoma were measured. Results: The concentration of VEGF in uterine vein and peripheral vein of hysteromyoma patients was 159.38±25.63pg/ml and 76.35±26.61pg/ml respectively. The peripheral vein VEGF level of control group was 42.53±29.47 pg/ml. The VEGF level in uterine vein was significant higher than peripheral vein (P=0.019) and the peripheral vein VEGF level of hysteromyoma patients was significant higher than normal control group (P=0.013), respectively. Conclusions: VEGF may mediate and enhance hysteromyoma growth.