子宫内膜癌组织中环氧化酶-2增强表达的临床意义

Overexpression of Cyctooxygenase-2 Gene and Its Clinical Significance in Human Endometrial Carcinoma.

目的:探讨环氧化酶2(COX2)在子宫内膜癌组织中的表达,COX2与雌激素受体(ER)表达的关系及COX2在子宫内膜癌发生发展中的意义。方法:应用RTPCR技术检测30例子宫内膜癌、相应癌旁正常内膜腺体及15例子宫良性肿瘤中COX2mRNA的表达。应用免疫组化方法测定30例内膜癌中ER表达情况。结果:COX2mRNA在30例子宫内膜癌组织中有28例表达水平明显增高,癌组织与正常内膜腺体或良性肿瘤间COX2的表达差异有显著性意义(P<0.05)。ER阳性组与ER阴性组的COX2表达差异有非常显著性意义(P<0.01)。结论:子宫内膜癌组织中COX2mRNA表达水平高于正常内膜腺体或子宫良性肿瘤,激素依赖型子宫内膜癌中COX2表达高于非激素依赖型内膜癌且其表达在子宫内膜癌的发生、发展中具有重要意义。

Objective: To study the expression of the cyclooxygenase-2 (COX-2) gene in endometrial carcinoma and its significance in the carcinogenesis and development of endometrial carcinoma. Methods: The expression of COX-2 mRNA was examined in cancerous tissues and adjacent normal endometrial tissue of 30 patients with endometrial carcinoma and in 15 benign uterine tumors by reverse transcription-polymerase chain reaction (RT-PCR). Estrogen receptors of 30 endometrial carcinomas were investigated by immunohistochemistry. Results: Enhanced expression of COX-2 was observed in ninety percent of endometrial carcinoma tissue. The difference of COX-2 mRNA expression among endometrial carcinoma, normal endometrial tissues, and benign tumor was significant (P<0.05) and the difference of that between estrogen receptor negative and positive was also observed (P<0.01). Conclusions:The expression level of COX-2 mRNA is obviously higher in endometrial carcinoma tissue than in normal tissue. The expression of COX-2 in hormone-dependent endometrial carcinoma is higher than that in non-hormone-dependent endometrial carcinoma. The overexpression of COX-2 may play a crucial role in the carcinogenesis and development of cancer in patients with endometrial carcinoma.