β地中海贫血的基因芯片检测

Gene chip detection of beta-thalassemia

目的探讨基因芯片在诊断β地中海贫血(简称β地贫)中的价值。方法对经筛查初诊的32例β地贫进行基因芯片检测,并与斑点杂交法进行比较分析。结果32例β地贫中,基因芯片技术检出32例,斑点杂交法检出30例,两者差别无统计学意义(P>0.05)。结论基因芯片技术与斑点杂交法在β地贫的诊断上可以互相代替;基因芯片技术具有快速、高效、自动化等特点,且简便、经济、省时省力,较斑点杂交法优越,宜提倡推广应用。

Objective To investigated the clinical application of gene chip techniques in the diagonsis of beta-thalassemia.Methods 32 cases of beta-thalassemia screened by routine methods were determined by using gene chip techniques and spot hybridization.Results All 32 patients suffering from beta-thalassemia were diagnosed by using gene chip techniques, in which 30 cases were determined by spot hybridization. There was no statistical difference between the two methods. Conclusion Gene chip techniques were simple and display much more advantages than spot hybridization such as taking short time, presenting high efficiency and costing less, etc.Therefore, spot hybridization can be replaced by gene chip techniques.