摘要
目的研究黄芪多糖对人早幼白血病HL-60细胞端粒酶活性的作用。方法不同浓度的黄芪多糖作用HL-60细胞后,MTT法测定细胞活性,计算细胞生长抑制率;TRAP-PCR-ELISA法测定端粒酶活性。结果黄芪多糖能抑制HL-60细胞的增殖,降低HL-60细胞的端粒酶活性,这两种作用均呈浓度和时间依赖性。结论黄芪多糖降低HL-60细胞端粒酶活性,可能是其诱导HL-60细胞凋亡的机制之一。
Objective To investigate the telomerase activity in HL-60 cells treated by Astragalus Polysaccharide.Methods The HL-60 cells were treated with Astragalus Polysaccharide and the cell inhibitory rate and telomerase activity were studied by MTT assay and TRAP-PCR-ELISA assay.Result The proliferation and telomerase activity of HL-60 cells treated with Astragalus Polysaccharide were significantly inhibited, and they depended on concentration and time.Conclusion Astragalus Polysaccharide could inhibit the telomerase activity of HL-60 cells, It might be one of regulatory mechanisms in the apoptosis of HL-60 cell.
出处
《河南肿瘤学杂志》
2005年第4期247-248,共2页
Henan Journal of Oncology
