摘要
目的:利用芯片技术探讨壬基酚(nonylphenol,NP)对F1代脑物质能量代谢调控相关基因表达影响。方法:NP染毒F0亲代母鼠,分别提取NP组和对照组F1代雄性2日龄大鼠的脑组织mRNA,分别用cy5和cy3逆转录标记,与BiostarR-40S基因表达谱芯片杂交,并对cy3、cy5荧光信号做扫描分析。结果:芯片结果显示实验组已知功能差异表达基因有16条,其中下调基因有13条,5条与物质能量代谢调控密切相关。结论:NP可通过多种途径影响机体物质能量代谢功能。
Objective:To explore the effects of nonylphenol on brain substance and energy metabolism associated gene expression profiles of F1 generation rats by means of microarray technique.Methods:The mRNA were extracted respectively from the brain of 2-day-old F1 generation rats whose F0 male generation were tested with and without nonylphenol,then reversely transcribed to cDNA and labeled with cy5 and cy3 fluorescence.Subsequently,the cDNA probes were hybridized to the BiostarR-40S cDNA microarray and the fluorescent signals of cy5 and cy3 were scaned and analyzed.Results:16 identified genes expressed differently,including 13 down-regulated in which 5 were related to substance and energy metabolism.Conclusion:Nonylphenol can effect the function of substance and energy metabolism of the male rats.
出处
《现代预防医学》
CAS
北大核心
2005年第8期874-876,共3页
Modern Preventive Medicine
基金
国家教育部西部高校高级访问学者基金(20023)
关键词
壬基酚
基因表达谱芯片
物质能量代谢调控相关基因
<Keyword>Nonylphenol
cDNA microarray
Substance and energy metabolism associated gene
