摘要
本研究以二乙基亚硝胺(DEN)诱发的大鼠肝癌为模型,通过病理组织化学、免疫 组化、生化、细胞周期动力学等观察,探讨养阴柔肝法对肝癌的防治作用。结果:1.中药组 癌前期的异型性变及成癌期的癌变程度较模型组轻,同时,其AFP阳性区、GGT阳性灶 及肝组织GGT、GST活性较低;2.中药组DNA合成期(S)细胞增多的幅度明显低于模型 组。提示:养阴柔肝法对DEN诱发动物肝癌有一定的阻抑作用;其作用可能与抑制肝细 胞DNA合成有关。
By means of the pathomorphology. histochemis-try, immunohistochemistry, biochemistry and cell cycle kinetics, the effect of the YangyitiRouganon preventing rat's hepatocarcinoma induced by Diethy-Inrosamine (DEN) was investigated. The results indicated that : 1) In the treated group, the allotypic lesion of the hepatocytes in precarcinoma and the degree of cancerrous convertion in hepatocarci-noma formation were slighter than in the controlld. Meanwhile, the positive area of GGT and AFP, activity of GGT and GST were reduced markedly in the liver. 2) In synthesis phase of DNA, the increased extent of the hepatocelluler number was greatly lower in the treated group than in the controll. It suggested that the nurishing yin and liver had effect on preventing rat's hepatocarcinoma induced by DEN. The effective mechanism of YRR perhaps related to inhibiting syntheses of intrahepatocellular DNA.
出处
《中国中医药科技》
CAS
1995年第3期11-14,共4页
Chinese Journal of Traditional Medical Science and Technology
基金
上海市高教局青年基金资助
