檵木叶抑菌活性成分提取分离及活性检测

Study on Eluates Bacteriostatic Active Composition and Bacteriostatic Activity from Loropetalum Chinese Leaves

用乙醇作提取溶剂,采用正交试验法对檵木叶抑菌活性成分提取条件进行研究,得最佳提取条件为A2B1C2,即60%乙醇、45℃、2h;选用四种树脂对该条件提取物的抑菌活性成分进行柱层析、梯度洗脱,以大孔吸附树脂、聚酰胺对檵木叶抑菌活性物分离效果较好,其水洗脱液抑菌活性最强;化学定性检识认为檵木叶含有多种抑菌活性成分,其主要活性成分可能为香豆素类。

The optimum condition for extracting antimicrobial substance by ethanol from the Loropetalum Chinese leaves wasinvestigated by orthogonal test. The optimum extracting conditions were: 60% ethanol, 45℃ and 2h. The results showed that theextracts could effectively inhibit the growth of bacteria. The bacteriostatic active eluates were separated by four resins. Thechemically identified results showed that the bacteriostatic active eluates were composed of coumarin, saponin, flavone andalkaloid.