摘要
从2-酮基-D-葡萄糖酸产生菌荧光假单胞菌(Pseudomonas fluorescens)A46的异常发酵液中分离到1种噬菌体,将其命名为KS461。透射电镜观察表明,KS461呈近球形,直径为52nm。以荧光假单胞菌A46为出发菌株,利用紫外诱变的方法,获得了4株对噬菌体KS461具有抗性的2-酮基-D-葡萄糖酸高产菌株,并研究了这些变异株的遗传稳定性。研究结果表明,经8次传代,这些变异株对噬菌体的抗性及其发酵产酸能力没有改变。
A phage, named KS461, was separated from the abnormal fermentation broth of Pseudomonas fluorescens A46 whichproduced 2-keto-D-gluconic acid. The KS461 was nearly spherical and 52nm in diameter as observed by transmission electronmicroscope. P. fluorescens A46 was mutagenized by using ultraviolet irradiation, and four phage-resistant mutants were obtainedwhich gave 2-keto-D-gluconic acid yield as high as that of the parent strain. Furthermore, the hereditary stability of the mutantswas investigated, and the results showed that the mutant resistance against the phage and 2-keto-D-gluconic acid producingability showed no change over 8 generations.
出处
《食品科学》
EI
CAS
CSCD
北大核心
2005年第7期67-70,共4页
Food Science
