摘要
本文通过抑菌活性分析,确定敏感菌,运用硅胶板层析技术,研究生物自显影对荸荠英提取物抑菌成分的活性跟踪方法:用200mg/ml荸荠英提取物丙酮溶液点样4μl,以二元溶剂氯仿:甲醇=8.5:1.5展开,贴板10min,37℃培养10h即可得到清晰的抑菌斑色谱图。应用该方法可将荸荠英粗提物乙酸乙酯部分分离得到六个活性组分。
The biological development technique was used to provide a qualitative analysis method for tracking bacferiostaficcomponents in puchiin. In the study, the Bacillus subtilis was regarded as indicator organism. The optimum conditions wereobtained as follows with 200mg/ml acetone solution extract: the volumn of sample was 4μl, the diffusion time and the culturetime were 10min and 10h respectively under the temperature of 37℃. Six different antibacterial points appeared with themixture of chloroform and carbinol in the ratio of 8.5 to 1.5.
出处
《食品科学》
EI
CAS
CSCD
北大核心
2005年第7期78-81,共4页
Food Science
