动物源食品中磺胺二甲嘧啶人工抗原的合成研究

Synthetic Study of Immunogen Sulphadimine Residue in Edible Foods

该文以丁二酸酐为间隔臂合成了人工半抗原,利用薄层层析法和高效液相色谱法进行半抗原的鉴定,通过活泼酯法合成了人工抗原,用Bradford法测定蛋白质含量,用紫外分光光度法测定偶联率。分析结果为:通过高效液相色谱法测定半抗原,显示合成成功,用紫外分光光度法测定其与BSA和OVAde偶联率分别为15和4。

In this paper, artificial haptene was synthesized taking succinic anhydride as spacer arm. Thin-Layer chromatographyand high performance liquid chromatography were applied to determinate it. Then, Reactive Ester Method was used tosynthesis complete antigen. After the level of protein measured with Bradford Method, conjμgated ratio was estimatedassociated with ultraviolet spectroscopy. The results indicated that the purity of hapene was high with the determination ofultraviolet spectroscopy and the conjugated ratio of BSA and OVA were l5 and 4 respectively.