龙须菜多糖抗突变和清除自由基作用的研究

Studies on Antimutagenic and the Free Radical Scavenging Effect of Polysaccharide from Gracilaria Lemaneiformis

目的探讨龙须菜多糖对小鼠抗突变作用的影响和对自由基的清除活性。方法采用热水提取、乙醇沉淀、三氯乙酸除蛋白等制备龙须菜粗多糖。观察小鼠经口服该粗多糖对环磷酰胺(CP)诱发的骨髓嗜多染红细胞微核和精子畸变的抑制作用,以及多糖对Fenton体系氧化产生的·OH和邻苯三酚自氧化产生的O2·的清除能力。结果该粗多糖在4g/kg剂量,对CP诱发的微核抑制率达89.8%、并能显著地抑制精子畸变(p<0.05);当1.1mg添加量时,对·OH的清除能力与同剂量苯甲酸接近;此外对O2·自由基也有一定的清除能力。结论龙须菜粗多糖有显著的抗突变能力且存在着量效关系;对体外氧化产生的自由基也有较强清除作用。--

Objective:To research the effect of anti-mutation in mice and the free radical scavenging activity of crudepolysaccharide from Gracilaria Lemaneiformis. Methods: The crude polysaccharid was extracted from Gracilaria Lemaneiformisby hot water, alcohol-precipitating and protein-depositing with chlor-acetic acid. The inhibitory effect was studied on mouse bonemarrow micronucleus and abnormal sperm induced by cyclophosphamide (cp) and the scavenging activities on· OH of Fentonreaction and O2·produced by pyrogallic acid of crude polysaccharide. Results:At the dose of 4 g/kg, the inhibitory ratio ofmicronucleus was 89.8%; It can significantly inhibit the aberrance of sperm (p<0.05). At the dose of 1.1 mg, the scavengingability on· OH was close to benzoic acid, while it also had the ability to scavenge O2·. Conclusion: The crude polysaccharidethe ability of anti-mutation and the effects of scavenging free radical oxidated out of the body.