可溶性淀粉作为手性选择剂对西酞普兰的毛细管电泳手性拆分

Chiral Separation of Citalopram by Capillary Electrophoresis with Water-soluble Amylose as Chiral Selector

目的:以可溶性淀粉作为手性选择剂,研究抗抑郁药西酞普兰的毛细管电泳手性拆分方法。方法:对手性选择剂的种类和浓度、缓冲溶液的浓度和pH以及分离电压等进行选择与优化。电泳条件为:石英毛细管柱内径50μm,总长56.5 cm,有效长度 44.0 cm;紫外检测波长239nm;电迁移进样10 kV×3s;温度20℃。结果:在含2.0%(w/w)可溶性淀粉、60 mmol·L-1磷酸盐 (pH 3.0)的缓冲溶液中,分离电压15 kV时,西酞普兰对映体达到基线分离,分离度为1.8。结论:采用毛细管电泳方法可以分离分析西酞普兰对映体,建立了西酞普兰对映体的毛细管电泳手性拆分方法。

Objective:Chiral separation of citalopram was performed by capillary zone electrophoresis on a fused silica capillary with water - soluble amylose as chiral selector. Methods:The effects of type and concentration of chiral selector, concentration and pH of background electrolyte, separation voltage used on the separation were investigated respectively. The separation was performed at 20℃ on a fused - silica capillary of 50 μm× 56.5 cm,effective length of 44. 0 cm. The detection wavelength was 239 nm. Sample was injected into capillary by electromigration at 10 kV for 3 seconds. Results:In the running buffer composed of 60 mmol·L-1 phosphate containing 2. 0% (w/ w) water - soluble amylose,at pH 3.0,and at the applied high voltage of 15 kV,the citalopram's enantiomers could be well separated. The resolution was 1.8. Conclusion:Good resolution of citalopram enantiomers were achieved under the optimal operation conditions.