高效液相色谱法测定阿立哌唑的含量

HPLC Determination the Content of Aripiprazole

目的:建立高效液相色谱法测定阿立哌唑原料药的含量。方法:采用Hypersil C18色谱柱(150 mm×4.6 mm,5μm),流动相:甲醇-磷酸盐缓冲溶液(20 mmol·L-1的磷酸二氢钾溶液,pH 6.3)(62:38),流速:1.0 mL·min-1,检测波长:257 nm。结果:阿立哌唑在0.1-20μg范围内线性关系良好(r=0.9999),检测限5×10-3μg。阿立哌唑与其有关物质的分离良好。结论:该法专属性强,结果准确,重现性好,可用作阿立哌唑含量测定和有关物质检查的方法。

Objective:To establish an HPLC method for determination the content of aripiprazole. Method:A Hypersil C18column(150mm×4.6 mm,5μm) was used with the mobile phase of methanol - phosphate buffer solution (pH6.3) (62:38). The flow rate was 1.0mL·min-1 and the detection wavelength was 257 nm. Result:The calibration curves were linear between 0.1-20μg(r=0.9999),the detection limit was 5×10-3μg. Conclusion:The method is simple,rapid,accurate and suitable for determination the content of aripiprazole.