气相色谱法测定鼻塞通喷雾剂中乙酸菊烯酯的含量

GC Determination of Chrysanthenyl Acetate in Bisaitong Spray

目的:建立鼻塞通喷雾剂中乙酸菊烯酯含量的气相色谱测定法。方法:采用气相色谱外标法。以2%OV-17为固定相,玻璃填充柱,氮气为载气,柱温由70℃以8℃·min-1升至130℃,FID检测器。结果:在该色谱条件下,制剂样品中的乙酸菊烯酯成分能得到良好的分离。加样回收率为97.70%(n=5)。用所建立的方法测得3批鼻塞通喷雾剂中乙酸菊烯酯的含量分别为1.79,1.38,1.90 mg·mL-1;并测定了主要原料鹅不食草挥发油中乙酸菊烯酯的含量,结果满意。结论:本法灵敏、准确、重现性好,可用于控制该制剂的质量。

Objective: To establish the assay of chrysanthenyl acetate in Bisaitong spray by GC. Method: The external standard GC method was applied. The GC system consisted of glass column with 2% 0V-17 as the stationary phase, nitrogen as the carrier gas, column temperature 70℃,8℃·min-1 , 130℃, and FID as the detector. Results:Chrysanthenyl acetate peak in the chromatogram of the preparation has got satisfying resolution under the chromatographic condition. The methodology study has been carried out,the mean recovery was 97.70% (n=5). The contents of chrysanthenyl acetate in three lots of the preparation were 1.79,1.38,1.90 mg·mL-1 respectively. The contents of chrysanthenyl acetate in the essential oils of Centipeda minima (L.) A. Br. et Aschers(the active material of the preparation) were also determined with satisfying results. Conclusion:The method is sensitive, accurate and reproducible, and it can be used to control the quality of the preparation.