缬草环烯醚萜抗肿瘤作用的实验研究

Experimental study of anti-tumor effect of valerian iridoids

目的探索缬草环烯醚萜的抗肿瘤作用。方法对从缬草中提取分离的总环烯醚萜类物质及其中的环烯醚萜甙与环烯醚萜酯进行体内(小鼠移植性肿瘤)、体外(噻唑蓝染色法)抗肿瘤活性研究。结果缬草环烯醚萜甙与环烯醚萜酯对K562,HL60,U937,HepG2和Hale细胞株具有突出的细胞毒作用,对小鼠在体的EAC(腹水型)、S180(实体型)呈现出明显抑瘤作用;其抗癌活性强度为双烯键结构的环烯醚萜酯>单烯键结构的环烯醚萜酯>环烯醚萜甙。结论缬草环烯醚萜类物质具有显著的细胞毒与抗肿瘤作用,尤其是环烯醚萜酯。

Objective It is to explore the anti-tumor effect of valerian iridoids. Methods The iridoids were extracted and isolated from valerian. The pan-iridoids, the iridoid glycosides and the iridoidates were observed on the anti-tumor active in vitro (MTT method) and in vivo (grafting tumors in mice). Results The valerian iridoids, the iridoidates and the iridoid glycosides had strong cytotoxin effects on K_ 562 , HL_ 60 , U_ 937 , HepG_2 and Hale cell. The compound of the iridoidates and the iridoid glycosides had direct curative effect on EAC (ascites), S_ 180 (entity) in mice. The intensity of anti-tumor activity was the iridoids with conjugate-bonds > the iridoids with mono-alkene > the iridoid glycosides. Conclusion Valerian iridoids have obvious cytotoxic and anti-tumor effect, especially the iridoidates.