乳腺癌与结肠癌衍生的免疫抑制因子及其特性的初步研究

Preliminary Study on Tumor-Derived Immunosuppressive Factor and Its Characteristics

观察乳腺癌与结肠癌组织培养上清液对植物血凝素（ＰＨＡ）诱导的患者及正常人外周血淋巴细胞（ＰＢＬ）增殖和白细胞介素２（ＩＬ－２）产量的影响，并对该上清液中的免疫抑制因子（ＴＤＳＦ）的理化性质作了初步研究。结果表明，两种癌组织培养上清液对ＰＨＡ诱导的患者本人及健康人的Ｔ细胞增殖均有明显抑制作用，且呈剂量依赖关系，而对ＩＬ－２产量无明显影响。理化特性研究见乳腺癌上清液含有两种活性成分，一种为对热及胰酶敏感的蛋白质，另一种为性质稳定的非蛋白质。

By using supernatant from cultures of tumor tissues of breast cancer and colon cancer, the effects of tumor-derived immunosuppressive factors(TDSF) on PHA-induced proliferation and IL-2 prduction have been investigated. The results showed that TDSF inhibited lymphocyte proliferation of cancer patients and healthy donors,but gave rise to no effect on IL-2 production. TDSF from breast cancer tissue contained 2 components that affected lymphocyte proliferation.One Was a protein With high absorption at 280um and was sensitive to heat and trypsin. The other one, most likely was a non-protein substance with very low absorption at 280urn and was heat stable and trysin-resistant.