人卵巢癌细胞分泌转化生长因子－β对外周血单个核细胞增殖的影响

Effect of Ovarian Cancer PrOduced-Transforming Growth Factor-βon Phytohemagglutinin and Interleukin-2-Inducing Proliferatlon of Peripheral Blood Mononuclear Cells

观察人卵巢癌细胞株ＣＯＣ＿１和ＣＯＣ＿２来源的患者癌性腹水（ＡＳ＿１，ＡＳ＿２）、癌细胞无血清条件培养液（ＳＦＣＭ＿１，ＳＦＣＭ＿２）及荷瘤裸鼠血清（ＮＳ＿１，ＮＳ＿２）对植物血凝素（ＰＩＩＡ）和白细胞介素－２（ＩＬ－２）诱导的外周血单个核细胞（ＰＢＭＣ）增殖的作用，及与转化生长因子－β（ＴＧＦ－β）的关系。结果：ＡＳ＿１和ＡＳ＿２对ＰＢＭＣ增殖有剂量依赖性抑制作用。ＳＦＣＭ和ＮＳ也具有明显抑制ＰＢＭＣ增殖的作用；但在浓度很低时，ＳＦＣＭ＿２对ＰＨＡ诱导，ＳＦＣＭ＿１、ＮＳ＿１和ＮＳ＿２对ＩＬ－２诱导的ＰＢＭＣ增殖，具有一定促进作用。上述作用特点与ＴＧＦ－β作用相似，并可被抗ＴＧＦ－β中和抗体部分阻断。提示：人卵巢癌细胞可通过产生ＴＧＦ－β，抑制宿主免疫。

bjectives Our previous observation has demonstrated that human ovarian cell lines COC_1 and COC_2 are able to secrete transforming growth factor-beta (TGF-β) in serum-free culture, The effect of ovarian cancer prOduced-TGF-β was observed on phytohemagglutinin and interleukin-2-inducing prolif- eration of peripheral blood mononuclear cells. Methods The effects of COC_1 and COC_2 from patients' ascitic fluid (AS_1, AS_2), the serum-free conditioned medium (SFCM_1, SFCM_2) , and COC_1- and COC_2- bearing nude mice sera (NS_1, NS_2) on phytohemagglutinin ( PHA) and interleukin-2 (IL-2)-inducing peripheral blood mononuclear cell(PBMC) proliferation and their correlation with TGF-βwere observed and compared. Results It was shown that AS, SFCM and NS possessed inhibitory effects on PBMC prolifera- tion. But in very low concentrations,PHA-induced by SFCM2 andIL-2-induced by SFCM1as well as by NS_1 and NS_2 exerted promotive effects on PBMC proliferation. The above-mentioned SFCM effects similar to that of TGF-β, might be partially blocked by anti-TGF-β antibody. Conclusions The present results suggest that ovarian cancer cells may inhibit the host anti-tumor immunity through secreting TGF-β, which may be associated with the highly malignant biological be- havior of ovarian cancer and the threatening progressiveness of its clinical course.