喉癌患者血清可溶性白细胞介素2受体和肿瘤坏死因子的检测及意义

Role of Determination of Serum Levels of sIL-2R and TNF in Laryngocarcinoma Patients

采用双抗体夹心ＥＬＩＳＡ方法，对正常人、声带息肉病人及喉鳞状细胞癌病人的血清可溶性白细胞介素２受体（ｓＩＬ—２Ｒ）和肿瘤坏死因子（ＴＮＦ）水平进行检测。结果表明，喉癌患者血清ＳＩＬ－２Ｒ水平和ＴＮＦ活性均明显高于正常人及声带息肉病人，差异非常显著（Ｐ＜０．０１，Ｐ＜０．００１）。且血清ｓＩＬ－２Ｒ水平与喉癌的病理分化程度、临床分型、分期及手术前后具有明显的相关趋势，ＴＮＦ则不明显。提示：动态观察血清ｓＩＬ－２Ｒ及ＴＮＦ的变化，可作为分析体内抗肿瘤效应、评价疗效及监测预后的参考指标。

A two layer antibody sandwich enzyme-linked immunosorbent assay(ELISA)was used to measure serum soluble interleukin-2 receptor (sIL-2R)and tumor necrosis factor(TNF)levels in 67 patients with laryngocarcinoma and 19 cases of vocal cord polyp and 35 normal subjects as control. The results showed that the sIL-2R and TNF levels of the patients with laryngocarcinoma were much higher than those of the vocal cord Polyp Patients as well as healthy individuals (P<0.01).Furthermore,the Serum sIL-2R levels of the patients with laryngocarcinoma were intimately associated with cellular differentiation, histological types and stages of disease and whether pre- or post-surgery, however no such associations were observed with serum TNF level.Consequently,It Is clear that alteration In the serum sIL-2R and TNF levels can be regarded as indicators of anti-tumor effect and is useful in the assessment of treatment efficacy and prognosis of the disease.