EB病毒编码的RNA及EB病毒潜在膜蛋白在中线T淋巴瘤中的表达

Expression of Epstein-Barr virus encoded small RNA-1 and latent membrane protein-1 in midline T-cell lymphomas

应用免疫组化和原位杂交技术及抗ＥＢ病毒潜在膜蛋白（ＬＭＰ－１）单克隆抗体和ＥＢ病毒编码的ＲＮＡ（ＥＢＥＲ－１）探针对９例中线Ｔ淋巴瘤（ＭＴＬ）进行了ＥＢ病毒（ＥＢＶ）检测。结果显示：８例肿瘤细胞核ＥＢＥＲ－１阳性；７例肿瘤细胞膜和胞浆ＬＭＰ－１阳性。结果表明：（１）ＥＢＶ与我国的ＭＴＬ存在密切关系，很可能在其发病中起着重要作用；（２）ＥＢＶ在ＭＴＬ的检出率高于全身其它部位和其它类型的周围型Ｔ淋巴瘤；（３）ＥＢＥＲ－１原位杂交和ＬＭＰ－１免疫组化在检测ＭＴＬ中ＥＢＶ方面都很敏感、可靠，而后者更经济简便。

ine cases of midline T-cell lymphoma(MTL)were studied for expression of Epstein-Barr virus(EBV)enceded small RNA-1(EBER-1) and EBV latent membrane proteinl (LMP-1) using in situ hvbridization and immunohistochemistry respectively.EBER-1 was detected in nuclei of tumor cells in8/9 cases of MTL and LMP-1 was found on membrane and in cytoplasm of tumor cells in 7/9 cases of MTL.The results show:(1)There is a strong association between EBV and MTL. EBV may play an important role in the pathogenesis of MTL,(2) The detection frequency of EBV in MTL is higher than peripheral T-cell lymphoma from other sites of the bedy.(3)EBER-1 in situ hybridization and LMP-1 immunohistochemistry are sensitive and reliable in detection of EBV in MTL and the latter is more eco-nomic andconvenient.