c－erbB－2蛋白和表皮生长因子受体在肝脏病变中的表达

Exprcssion of c-erbB-2 protein and EGF reccptor in hepatitis B,cirrhosis and hepatocellular carcinoma

对１８４例乙型肝炎、肝硬变和肝细胞癌（ＨＣＣ）及２９例正常肝组织的标本作了ＡＢＣ法染色，观察其ｃ－ｅｒｂＢ－２蛋白和表皮生长因子受体（ＥＧＦＲ）的表达情况。３６％（４８／１３４）的慢性肝炎、肝硬变和ＨＣＣ组织中有ＥＧＦＲ表达，主要定位于血窦内皮。良、恶性病变肝组织之间在ＥＧＦＲ表达强度上无显著差别，提示ＥＧＦＲ可能与慢性肝脏病变中血窦内皮的增生有关。在正常肝，仅少数标本（５／２９）中见到ｃ－ｅｒｂＢ－２微弱表达；在ＨＢＶ相关的慢性肝脏病变，所有标本中均检测到ｃ－ｅｒｂＢ－２蛋白，主要定位于肝小多角细胞（ＳＰＬＣ）、小细胞性不典型增生（ＳＣＤ）及小管状化生（ＤＭ）的肝细胞；ＨＣＣ细胞中ｃ－ｅｒｂＢ－２蛋白阳性较弱。这提示ｃ－ｅｒｂＢ－２基因的活化与人ＨＣＣ发生有关。其作用机制可能是促进ＳＰＬＣ向ＳＣＤ的转化及促进ＳＣＤ的进展。ｃ－ｅｒｂＢ－２与ＨＢｘＡｇ表达的密切关联提示这种原癌基因的活化可能也与ＨＢＶＸ基因有关。

The expressions of c-erbB-2 oncogene and epidermal growth factor receptor(EGFR)were investi-gated immunohistochemically in specimens from 184 cases of hepatitis B,cirrhosis and hepatocellular-carcinoma (HCC)and 29 normal liver specimens. EGFR was expressed in 36%(48/134)of the hepato-cellular carcinoma and chronic liver disorder specimens and it was immunolocalized mainly in the sinu-soidal endothelial cells.No significant difference was found between EGFR expression in HCC and in be-nign chronic liver disorders.These results indicate that EGFR may have some role in the proliferation of the sinusoidal epithelial cells in chronic liver disease,Low level c-erbB-2 expression was observed in 5/29(17%)of normal liver specimens.In chronic hepatitis B and liver cirrhosis, its expression was found in all speciments,c-erbB-2 protein was immunolocalized mainly in small polygonal liver cells (SPLCs) and hepatocytes in small-cell dysplasia (SCD)and in ductular metaplasia(DM);c-erbB-2 expression in HCC cells was found to be weaker than in SPLCs,the hepatocytes in SCD and in DM. These results indicate that activated c-erbB-2 oncogene may have a role in human HCC genesis through promoting the develop-ment of SCD from SPLC proliferation and the progression of SCD. The close relat ion between the ex-pression of c-erbB-2 and HBxAg imply that the activation of c-erbB-2 in human liver tissues may be re-lated to HBV X gene.