缺氧对肺动脉内皮细胞PDGF基因及PDGF－B链蛋白表达的影响

Effetts of hypexia on the expression of platelet-derived growth factor(PDGF) gene and PDGF-B chain protein in porcine pulmonary artery endothelial cells

应用核酸杂交和定量免疫细胞化学分析技术研究了缺氧对猪肺动脉内皮细胞血小板源性生长因子ＰＤＧＦ基因及ＰＤＧＦ－Ｂ链蛋白表达的影响。结果表明，缺氧能明显增强肺动脉内皮细胞ＰＤＧＦ＿Ｂ链ｍＲＮＡ的表达，而ＰＤＧＦ－Ａ链ｍＲＮＡ表达水平无明显改变。免疫细胞化学定量分析也证实ＰＤＧＦ－Ｂ链蛋白染色比正常对照明显增强，与ＰＤＧＦ－Ｂ链基因表达的改变基本一致。提示缺氧可以刺激肺动脉内皮细胞ＰＤＧＦ的表达。ＰＤＦＧ在缺氧引起的肺动脉高压血管构形改建中可能具有重要作用。

orthern blot,slot blot hybridization and quantitative immunocytochemistry analyses were used tostudy the effects of hypoxia on the expression of PDGF gene and PDCJF-B chain protein in cultured pul-monary artery endothelial cells(PAEC). Hypoxia(3%oxygen )significantly increased PDGF-B chainmRNA in PAEC,whereas mRNA level of PDGF-A chain was not increased significantly in comparisonwith normoxic control( 2l%oxygen ).PDGF-B chain immunoreactivity on PAEC exposed to hypoxiawas markedly stronger than that of normoxic PAEC and PDGF-B China immmunoreaction. was foundin cytoplasm and perinuclear region of the hypoxic PAEC,however,only in the nuclear region of nor-moxic PAEC,Increased expression of PDGF-B chain protein roughly paralleling that of PDGF-B mR-NA was also observed.These results suggest that hypoxia may stimulate the expression of PDGF-Bchain by PAEC in vivo and PDGF may play an important role in the vascular remOdeling of hypoxic pul-monary hypertension。