EB病毒感染及感染拷贝数与肺癌的关系

Tbe role of Epstein-Barr virus infection and infective copy number in pulmonary carcinogenesis

ＥＢ病毒与鼻咽癌、Ｂｕｒｋｉｔｔ淋巴瘤等恶性肿瘤的发生密切相关，但与肺癌的关系目前仍不清楚。我们收集８７例外科手术切除，同时含有肺癌及癌旁正常肺的石蜡包埋组织，用多聚酶链反应及斑点杂交技术对其中的ＥＢ病毒感染情况及感染的拷贝数进行检测。ＰＣＲ检测结果：肺癌５２例，对应癌旁肺组织３９例ＥＢ病毒阳性，统计学上无显著差异；斑点杂交结果：肺癌３８例，相应癌旁肺组织７例有ＥＢ病毒高拷贝感染，两者在统计学上存有非常显著的差异。结果提示：ＥＢ病毒在肺及肺癌组织中有较高的感染率，但肺癌组织中ＥＢ病毒感染拷贝数明显高于癌旁肺组织并有非常显著的差异；高拷贝数的ＥＢ病毒感染在肺组织细胞的恶性转化过程中可能起一定的作用。

o study the possible role of EBV in pulmonary carcinogenesis,surgically resected and paraffin-em-bedded pulmonary carcinoma specimens from 87 cases in which each specimen contained separable carci-noma tissue and normal lung tissue(twin pair) were collected to detect EBV infection and infective copynumber by polymerase chain reaction (PCR) and dot blot hybridization. of the 87 cases(174 samples),EBV genomic DNA sequences were positive in 52(59.8%)carcinoma tissue samples and 39 (44.8%)normal tissue samples by PCR. There were no significant differences in positive rates between the twotissues. By dot blot hybridization,a significant difference was found between carcinoma(43.7%)andnormal lung(8.0%)tissues. The results suggest that EBV infection may be common in both normaland carcinoma tissues,but carcinoma tissue contains higher EBV infective copy number than normal tis-sue. Infective copy number of EBV rather than simple EBV infection may play an important role in pul-monary carcinogenesis.