胃癌神经内分泌细胞CgAmRNA的原位杂交研究

Detection of CgA mRNA in neuroendocrine cells in gastric carcinoma by in situ hybridizatiion

应用ＣｈｒｏｍｏｇｒａｎｉｎＡ（ＣｇＡ）寡核苷酸探针原位杂交技术对１７例胃癌及癌旁粘膜的神经内分泌（ＮＥ）细胞进行观察。并与抗ＣｇＡ抗体免疫组化比较。结果发现１７例胃癌中５例有ＣｇＡｍＲＮＡ的表达，７例ＣｇＡ蛋白呈阳性表达，其中４例同步表达。上述结果表明胃癌组织中存在神经内分泌标志ＣｇＡｍＲＮＡ的表达，这类细胞不仅有储存分泌ＣｇＡ蛋白产物的能力，而且有ＣｇＡ基因水平的表达，具有合成该物质的能力。

resh gastric carcinoma specimens from 17 cases were collected.The neuroendocrine ( NE)cells in gastric carcinorTla(GC ) and gastric mucosaL adjacent to the carcinorna ( GMAC)were observed using in situ hybridization of chromogranin A(CgA)oligonucleotide probe and compared with imrnunohis-tochemistry of CgA antibody. 5 of the 17 cases of GC showed CgA mRNA positive expression and 7 of the 17 cases expressed CgA protein positively. The simuItaneous expression of mRNA and protein of CgA was present in 4 cases. The NE cells in GC not only can store and secrete CgA protein products but also possess the ability to synthesize NE products from gene expression.