卵巢上皮性肿瘤肠粘液抗原和癌胚抗原的免疫组织化学研究

An immunohistochemical study of intestinal mucinous antigeus and CEA in ovarian epithelial tumors

应用两种肠粘液抗原（ＬａｒｇｅＩｎｔｅｓｔｉｎａｌＭｕｃｉｎｏｕｓＡｎｔｉｇｅｎ，ＬＩＭＡ，ＳｍａｌｌＩｎｔｅｓｄｔｉｎａｌＡｎｔｉ－ｇｅｎ，ＳＩＭＡ）和ＣＥＡ对７８例卵巢上皮性肿瘤石蜡标本进行免疫组化染色。三种抗原在良性和交界性肿瘤中以腔缘型分布为主，可见恶性肿瘤腔缘、胞浆弥漫分布并随组织学分级的增高趋向于胞浆分布；三种抗原的阳性率在浆液性癌和粘液性癌中的差别不显著，但ＣＥＡ在浆液性癌中表达强度低于粘液性癌（Ｐ＜０．０５）；在子宫内膜样癌中三抗原之阳性率和表达强度均较低，故可以辅助鉴别浆液性癌、粘液性癌和官内膜样癌。ＣＥＡ和ＬＩＭＡ大量出现于癌细胞内的肿瘤更具侵袭性，易致肿瘤扩散转移，预后较差。

sing monoclonal antibodies specific to the large intestinal mucinous antigen(LIMA),small in-testinal mucinous antigen(SIMA)and CEA, the expression and localization of these proteins in 78 for-malin fixed paraffin embedded tissues from ovarian epithelial tumors were studied.The localization of LIMA, SIMA and CEA was associated with tumor features and differenciation. Positive stain of benign and borderline tumors were mostly located on the lumina border while malignancies were situated both on the lumina border and in the cytoplasm and tended to concentrate in the latter with the increase in histological grade. No difference in positive rate was seen between serous and mucinous carcinomas.However,staining intensity of CEA in serous carcinomas was lower than that in mucinous carcinomas and the expression and intensity in endometrioid carcinomas were low. On the other hand,positive CEA and LIMA was associated with a significantly worse survival.