血管性痴呆小鼠大脑皮质NOS活性及nNOS蛋白表达的改变

Changes in activity of NOS and expression of nNOS in cerebral cortex of mice with vascular dementia

目的观察血管性痴呆小鼠大脑皮质一氧化氮合酶(NOS)和神经元型一氧化氮合酶(nNOS)阳性神经元的变化,探讨血管性痴呆的发生机制。方法复制小鼠血管性痴呆模型,利用Y-迷宫检测血管性痴呆模型小鼠学习记忆能力,采用NADPH-d组织化学和ABC免疫组织化学方法,研究血管性痴呆小鼠与正常小鼠大脑皮质NOS和nNOS阳性神经元数量的变化。结果血管性痴呆小鼠比正常小鼠Y-迷宫学习记忆训练次数明显增多,差异有统计学意义(P<0.01);大脑皮质NOS和nNOS阳性神经元的数量明显增多,差异有统计学意义(P<0.05)。结论血管性痴呆的发生可能与大脑皮质NOS和nNOS阳性神经元的数量明显增多有关。

Objective To observe the changes of vigor of nitric oxide synthase (NOS) and the expression level of neuronal nitric oxide synthase (nNOS) in the cerebral cortex of mice with vascular dementia and investigate the mechanism of vascular dementia formation.Methods A model of vascular dementia in mice was developed.NADPH-d histochemistry and ABC immunohistochemistry were used to investigate the changes in NOS in different groups.Y-maze test was used to observe the changes in the learning and memorizing abilities.Results Compared with the control group, the learning and memorizing abilities of the vascular dementia group were significantly decreased; the NOS positive and nNOS positive neurons in cerebral cortex were significantly increased.Conclusions The formation of vascular dementia is probably related to the increase in number of NOS positive and nNOS positive neurons in cerebral cortex.