基质细胞衍生因子-1和受体CXCR4在急性白血病和恶性淋巴瘤中的表达

The expression and clinical significance of stromal cell-derived factor-1 and CXCR4 in acute leukemia and malignant lymphoma

目的分析急性白血病(AL)和恶性淋巴瘤(ML)患者外周血血清基质细胞衍生因子1(SDF1)及其骨髓单个核细胞(MNC)表面特异性受体CXCR4的表达,并进行对照研究。方法ELISA法检测血清中SDF1表达,流式细胞术检测骨髓MNC表面CXCR4的表达。结果26例AL患者包含9例急性淋巴细胞白血病和17例急性非淋巴细胞白血病,其SDF1、CXCR4表达分别为(7115.8±946.5)ng/L、(77.2±9.7)%和(4642.2±1146.8)ng/L、(38.9±11.0)%;18例ML患者包含5例霍奇金淋巴瘤和13例非霍奇金淋巴瘤,其SDF1、CXCR4表达分别为(3728.9±690.9)ng/L、(9.2±2.7)%和(4442.1±1073.0)ng/L、(8.5±2.4)%,上述各组SDF1/CXCR4表达均高于对照组水平(2369.3±966.5)ng/L、(2.7±1.5)%(P<0.01)。有髓外浸润的AL患者其SDF1/CXCR4的表达水平高于同组其他患者,有广泛浸润的ML患者其SDF1的表达水平高于同组其他患者。结论CXCR4/SDF1的高表达与AL和ML的发病及其浸润特性存在一定联系,为上述肿瘤的治疗提供了新思路。

Objective To analyze the expression of stromal cell derivd factor-1(SDF-1) and its functional chemokine receptor CXCR4 in patients with acute leukemia and malignant lymphoma. Methods(CXCR4) expressed on the membrane surface of marrow mononuclear cells was enumerated with 3-color flow cytometry. The serum level of SDF-1 was determined with ELISA assay. Results The expression of SDF-1 and CXCR4 in acute leukemia for ALL group,the expression of SDF-1 and CXCR4 is (7115.8±946.5) (ng/L) and (77.2±9.7)%;for ANLL group,the expression is (4642.2±1146.8) ng/L、(38.9±11.0) % and malignant lymphoma for HD group,the expression of SDF-1 and CXCR4 is (3728.9±690.9) ng/L and (9.2±2.7)%;for NHL group,the expression is (4442.1±1073.0) ng/L and (8.5±2.4)% patients were higher than that in controls the expression of SDF-1 and CXCR4 is (2369.3±966.5) ng/L and ((2.7±1.5)%) (P<0.01). In the leukemia group, the patients with extra-marrow infiltration have higher expression of SDF-1/CXCR4 than those without and the patients; in the lymphoma group. Conclusions(The high) expression of SDF-1 and CXCR4 is somewhat correlatied with the pathogenetic process and infiltration characteristics of acute leukemia and malignant lymphoma. This can provide a new method to cure the above-meetioned hematologic tumors.