超声对小鼠胚泡和子宫内膜细胞及假孕鼠再孕的影响

Effects of Ultrasound on Mouse Blastocyst and Endometrial Epithelial Cells and on Pregnant Capacity of Pseudopregnant Mouse

观察子宫内膜上皮细胞和胚泡的生长发育情况并对治疗声强超声作用于两种不同来源组织细胞的影响进行比较性研究。同时，也观察治疗声强超声辐照假孕鼠后对其再次妊娠是否有影响。实验结果显示：强度为１２Ｗ／ｃｍ２超声辐照小鼠妊娠第４天的子宫９０ｓ后，高体培养胚泡的死亡率为８１．９３％，而培养的子宫内膜上皮细胞生长良好，培养第３天的上皮细胞内ＤＮＡ组化测定与假照组在统计学上无差异。用该剂量辐照假孕第４天小鼠子宫后对该鼠的再孕无明显影响。结果说明；胚泡较子宫内膜上皮细胞对超声波更敏感，同时也说明在一定范围内的治疗剂量超声对假孕鼠的再孕亦无明显影响。

This paper observed epithelial cells and blastocyst growth and development and made a comparative research on the effect of ultrasound on the cells from these two different tissues. At the same time, it was also to observe whether the application of the therapeutical dosage of ultrasound on mouse uterus of pseudopregnancy affected the future gestation. Results: The experiment results revealed that after the exposure of the mouse uterus to the ultrasonic wave of an interrisity of 12W/cm2× 90s on the 4th day of gestation, the in vitro cultured blastocyst displayed the death rate of 81.93%,while the cultured endometrial epithelial cells showed in normal growth, and on significant difference was found between the experimental and the sham-irradiation groups in the DNA histochemical test of the endometrial cells conducted on the 3rd day of culture. The application of same dosage (12W/cm2 ×90s) of ultrasound on mouse uterus on the 4th day of pseudopregnancy was proved to have no effect on future gestation. Such result indicates that embryos are more sensitive to ultrasonic irradiation than endometrial epithelial cells of uterus and that the application of within the special range of the therapeutical dosage of ultrasound on mouse uterus of pseudopregnancy has no effect on future gestation.