血清有机膦酸酯酶活性在慢性肝病中的变化及其与肝纤维化的相关性研究

ACTIVITY OF SERUM PHOSPHONATE ESTERASE IN CHRONIC LIVER DISEASES AND ITS RELATIONSHIP TO HEPATIC FIBROSIS

为探讨血清有机膦酸醋酶（ＰＥ）活性与肝纤维化的关系，用荧光分光法测定了７７例慢性肝病患者及实验诱发的肝纤维化大鼠血清ＰＥ活性变化。发现慢性肝病时血清ＰＥ活性明显升高，其升高程度依次为肝硬化＞原发性肝癌＞慢性活动性肝炎＞慢性迁延性肝炎。与血清Ⅲ型前胶原氨基末端肽（ＰⅢＰ）水平变化呈正比。肝纤维化大鼠血清ＰＥ活性也随肝纤维化程度加重而逐渐升高，与肝纤维化积分及血清Ⅲ型前胶原（ＰＣⅢ）、透明质酸（ＨＡ）变化呈显著正相关，相关系数分别为０．９６４，０．９９８与０．９６７，均Ｐ＜０．０１。说明血清ＰＥ活性与肝纤维化程度间有明显关系，可作为肝纤维化的血清学诊断指标之一。

The purpose of the study was to investigate the relationship between serum pho-sphonate esterase(PE)and liver fibrosis. Serum PE activities in 77 patients withchronic liyer diseases and in rats with liver fibrosis induced by carbon tetrachloridewere measured by fluronic method.Serum PE activities were increased in variouschronic liver diseases(cirrhosis>primary liver cancer>chronic active hepatitis>chronicpersistent hepatitis), which was consistant with the changes of serum type III proco-llagen peptides(PIIIP).Serum PE activities in rats with liver fibrosis were also signi-ficantly increased. The degree of Serum PE enhancement was related to the liver colla-gen deposition, serum type III procollagen(PcIII) and hyaluronic acid(HA). Therelated coefficients Were 0.964,0.998 and 0.967,respectively. Serum PE activity ispositively related to hepatic fibrogenesis and it can be used as a serum marker in thediagnosis of liver fibrosis.