HBV感染的单核细胞分泌TNFα和PGE_2的改变及其意义探讨

THE CHANGE OF TNFα AND PGE2 SECRETED BY HBV-INFECTED MONOCYTES AND ITS POSIBLESIGNIFICANCE IN HEPATITIS PATIENTS

本文作者用聚合酶链反应（ＰＣＲ）技术检测了７０例慢性乙型肝炎患者外周血单核细胞内ＨＢｖＤＮＡ，并用双抗体夹心法、放射免疫分析法，分别检测了单核细胞培养上清肿瘤坏死因子α（ＴＮＦα）、前列腺素Ｅ２（ＰＧＥ２）含量，结果显示，单核细胞内ＨＢＶＤＮＡ阳性者培养上清ＴＮＦα含量显著增高，ＰＦＥ２含量显著下降。临床病情越重，单核细胞内ＡＢＶＤＮＡ检出率越高，其培养上清ＴＮＦα含量越高，ＰＧＥ２含量越低。提示ＨＢＶ感染单核细胞可导致其分泌功能异常，从而加重肝组织损害。

HBV DNA in peripheral blood monocytes from 70 patients with chronic hepatitisB were detected by the polymerase chain reaction(PCR) technique. The tumor necrosisfactor alpha(TNFα )and prostaglandin E2(PGE2)in monocyte culture supernatantwere measured by ELISA or radioimmunoassay. It was found that HBV-DNA positivemonocytes produced much more TNFα and significant less PGE2 than HBV-DNA nega-tive ones. The more serious the hepatitis , the higher the percentage of HBV-DNA posi-tive monocytes and the content of TNFα in monocyte culture supernatan, but the lowerthe content of PGE2 in monocyte culture supernatant.These data suggested that theinfection of monocytes with HBV might result in abnormality of monocytes , secretoryfuction,this might have something to do with the damage of hepatic cells of patientswith chronic hepatits B.