基于超短脉冲激光光谱技术诊断癌症的研究

Study of Diagnosis Cancer Based on Ultrashort Pulses Laser Spectral Technique

目的:研究用于癌症诊断与治疗的光敏剂血卟啉(HPD)的超快光动力学过程;方法:采用超短脉冲激光光谱技术和皮秒时间相关单光子计数系统,测量经血卟啉培养的活体癌细胞与正常细胞的荧光光谱、荧光寿命特性及荧光峰值强度随时间变化曲线,并测量单一细胞内部不同位置的荧光寿命特性;结果:癌细胞样品在645nm处具有特有的光谱谱峰;癌细胞样品荧光寿命的快成分约150ps慢成分约1200ps,而正常细胞样品快成分约300ps慢成分约2500ps;癌细胞样品的荧光峰值强度经12小时衰减约10%,而正常细胞样品衰减约55%;在细胞内部荧光寿命300ps的快成分十分显著,且中心部位血卟啉浓度最高;结论:癌细胞与正常细胞的荧光光谱、荧光寿命特性及荧光峰值强度随时间变化曲线相差十分明显,反映了癌细胞与正常细胞对血卟啉亲和性有显著的差异;测量结果确认了荧光光谱技术诊断与治疗癌症的可行性,并对发展超短脉冲激光光谱技术早期诊断与治疗癌症具有重要的指导意义和临床应用价值。

Objective: The object of this study was to ultrafast photodynamic processes of hematoporphyrin derivative(HPD); Methods: Fluorescence spectra and decay characteristics of normal and cancerous cells were measured using a ultrashort pulses laser spectral technique and picosecond time-correlated single-photon counting system; Results: Characteristic fluorescence spectra peak near 645nm of cancerous cells sample was observed, significant change in fluorescence spectra was found between normal and cancerous cells, Furthermore, fast part of cancerous cells fluorescence decay was approximately 150ps and slow part was approximately 1200ps, fast part of normal cells fluorescence decay was approximately 300ps and slow part was approximately 2500ps, it was varies in fluorescence decay. The measurement of fluorescence decay characteristics at different location inside the cells was performed by the same method. Conclusions: The results obtained can be used as an important basis for diagnosis cancer based on ultrashort pulses laser spectral technique.