动脉硬化病因学研究:巢式聚合酶链反应法测定冠心病患者外周血肺炎衣原体DNA

Etiology of arteriosclerosis:Detection of chlamydia pneumoniae DNA in peripheral blood of patients with coronary heart disease with nested polymerase chain reaction

目的:探讨冠心病患者外周血肺炎衣原体DNA测定的意义,以期发现动脉硬化发生的可能机制。方法:选择2001-12/2003-12广东医学院附属福田人民医院心内科住院冠心病患者145例,男88例,女57例。应用巢式聚合酶链反应法,测定145例冠心病患者外周血中DNA。结果:本组患者肺炎衣原体DNA阳性48例,阳性率33.1%,其中男性88例,阳性30例,阳性率为34.1%,女性57例,阳性17例,阳性率为29.8%,男女之间阳性率比较,差异无显著性意义(P>0.05)。结论:研究结果表明冠心病患者外周血中存在肺炎衣原体DNA,肺炎衣原体在动脉硬化发生过程中起着重要作用。

AIM:To study the significance of detection of chlamydia pneumoniae(Cpn) DNA in peripheral blood of patients with coronary heart disease(CHD),so as to find out the possible mechanism of the occurrence of arteriosclerosis. METHODS:Totally 145 CHD inpatients(88 males,57 females) were selected from the cardiological department of Futian People's Hospital affiliated to Guangdong Medical College from December 2001 to December 2003.The DNA in the peripheral blood of the 145 CHD patients was measured with the nested polymerase chain reaction(PCR). RESULTS:The Cpn DNA was positive in 48 cases,and the total positive rate was 33.1%.There was no significant difference in the positive rate between males(30/88,34.1%) and females(17/57,29.8%)(P >0.05). CONCLUSION:The results indicate that Cpn DNA can be detected in the peripheral blood of CHD patients,and Cpn plays an important role in the occurrence of atherosclerosis.