青藤碱和甲氨蝶呤对降植烷诱导大鼠关节炎发病率及关节炎症表现的影响

Effect of sinomenine and methotrexate on incidence of arthritis and its manifestations in rats with pristane induced arthritis

目的:观察青藤碱和甲氨蝶呤对降植烷诱导大鼠关节炎发病率及关节的炎症表现。方法:实验于2003-10/2004-05在大连医科大学病理生理学动物实验室完成。取SPF级近交系Lewis大鼠40只,雌性,分成5组,正常对照组,造模组,青藤碱组,甲氨蝶呤组及青藤碱组+甲氨蝶呤组,每组8只。除正常组外,每只大鼠尾根部位一次性皮内注射降植烷0.2mL。正常对照组大鼠每只皮内注射等体积的生理盐水。造模当天开始分组口服给药。正常组和模型组予10mL/(kg·d)生理盐水;青藤碱组予青藤碱120mg/(kg·d);甲氨蝶呤组予甲氨蝶呤每周3.8mg/kg;青藤碱+甲氨蝶呤组予青藤碱120mg/(kg·d)和甲氨蝶呤每周3.8mg/kg;建立关节炎动物模型大鼠32只,持续上述治疗8周,然后取右侧距小腿(踝)关节依次行X射线片及病理检查。结果:40只大鼠进入结果分析,无脱失。①模型组,青藤碱组降植烷诱导的关节炎发病率分别为62.5%,75.0%;甲氨蝶呤组与青藤碱+甲氨蝶呤组降植烷诱导的关节炎发病率均为12.5%。②青藤碱组与模型组的关节病理和X射线检查结果出现病变距小腿关节滑膜增生,炎细胞浸润,软骨面破坏;甲氨蝶呤组和青藤碱+甲氨蝶呤组无此表现。结论:青藤碱对降植烷诱导的关节炎大鼠发病可能无影响,甲氨蝶呤可抑制降植烷诱导的关节炎大鼠发病。

AIM: To observe the effect of sinomenine and methotrexate on incidence of arthritis and its manifestations in pristane induced arthritis rats. METHODS: The experiment was conducted in the Animal Experiment Laboratory of Pathophysiology, Dalian Medical University from October 2003 to May 2004. Forty SPF grade inbred line female Lewis rats were involved. All the animals were divided into 5 groups: normal control group, model group, sinomenine group, methotrexate group, sinomenine and methotrexate group, 8 rats in each group. All the animals excepted for those in the normal control group were performed subcutaneous injection of 0.2 mL pristane into the tail-head of the rats. The same volume of normal saline was injected into the rats in the normal control group. Medicine was given orally to the rats in the model group from the beginning of the modeling. 10 mL/(kg·d) normal saline was injected into the rats in the normal control group and model group; 120 mg/(kg·d)sinomenine was given to the rats in the sinomenine group; The rats in the methotrexate group were given methotrexate, 3.8 mg/kg each week; 120 mg/(kg·d)sinomenine and 3.8 mg/kg methotrexate each week were given to the rats in sinomenine and methotrexate group. Thirty-two arthritis animal models were established. After 8-week treatment, the right ankle joints of the animals were collected to perform X-ray and pathological examination. RESULTS: Forty rats entered the result analysis with no loss in the midway. ①The incidence of arthritis induced by pristine in the model group and sinomenine group was 62.5% and 75.0% respectively; and that in the methotrexate group and sinomenine and methotrexate group was both 12.5%;②The joint pathology and X-ray examination result in the sinomenine group and model group showed that the synovial membrane of ankle joint proliferated , inflammation cell infiltrated and the cartilage was destroyed; There were not such manifestations in the methotrexate group and sinomenine and methotrexate group. CONCLUSION: Sinomenine may not have effect on the onset of arthritis induced by pristine in rats. Methotrexate can inhibit the onset of arthritis induced by pristine.