原发性高血压血瘀证患者的热休克蛋白70基因及c-fos基因——基因表达与微观辨证的关联(英文)

Relationship between gene expression and micro-differentiation: heat-shock protein 70 and c-fos genes in blood stasis syndrome of primary hypertension

背景:c-fos和热休克蛋白70基因均为原发性高血压血瘀证患者密切相关基因,参与了原发性高血压血瘀证的发生发展,研究二者的相关性对于原发性高血压的防治有重要意义。目的:通过免疫组织化学方法检测原发性高血压血瘀证患者热休克蛋白70与c-fos基因表达水平,探讨其相关性。设计:病例分析。单位:暨南大学医学院中医系。对象:选择2002-03/2003-03山东中医药大学第二附属医院心血管内科住院和门诊确诊为原发性高血压患者148例。男80例,女68例。干预:入院第3天在晨起7:30～8:00抽取空腹静脉血2mL,进行热休克蛋白70及c-fos蛋白免疫组织化学检测,评估标准:阴性表达(-),未见棕黄色染色的细胞;阳性表达(+),每高倍视野阳性细胞数<20%;显性表达(++),每高倍视野阳性细胞数20%～50%;强阳性表达(+++),每高倍视野阳性细胞数>50%。并应用Ridit分析统计学方法对两者相关性进行分析。主要观察指标:热休克蛋白70与c-fos基因表达。结果:参加试验148例原发性高血压患者,所有患者血样均进入结果分析。①热休克蛋白70和c-fos基因表达均为阳性6例。②热休克蛋白70基因表达显性,c-fos基因表达阳性4例。③热休克蛋白70基因表达强阳性,c-fos基因表达阳性12例。④热休克蛋白70基因表达阳性,c-fos基因表达显性6例。⑤热休克蛋白70和c-fos基因表达均为显性19例。⑥热休克蛋白70基因表达强阳性,c-fos基因表达显性16例。⑦热休克蛋白70基因表达阳性,c-fos基因表达强阳性1例。⑧热休克蛋白70基因表达显性,c-fos基因表达强阳性28例。⑨热休克蛋白基因表达70和c-fos基因表达均为强阳性56例。⑩热休克蛋白70与c-fos基因表达阳性等级有显著相关性(R=0.2811～0.6446,P<0.01)。结论:在原发性高血压血瘀证中,热休克蛋白70和c-fos基因均有较高的表达,具有明显的相关性。

BACKGROUND: Both c-fos and heat-shock protein (HSP) 70 genes are closely relevant with and participate in the occurrence and development of blood stasis syndrome of primary hypertension. It is importance to study on their correlation for prevention of primary hypertension. OBJECTIVE: Immunohistochemical method was used to determine the expression of HSP70 and c-fos genes in blood stasis syndrome of primary hypertension so as to probe into their correlation. DESIGN: Case analysis. SETTING: Department of Traditional Chinese Medicine, Medical College of Jinan University. PARTICIPANTS: Totally 148 cases of primary hypertension were selected, diagnosed in ward and clinic of Department of Cardiac Vascular Internal Medicine of Second Affiliated Hospital of Shandong University of Traditional Chinese Medicine from March 2002 to march 2003, of which, 80 cases were male and 48 cases were female. INTERVENTIONS: At 7:30 to 8:00 in the morning on the 3rd of hospitalization, 2 mL intravenous blood was collected in fasting for immunohistochemical determination of HSP70 and c-fos genes. Evaluative standards: negative expression (-), no brown-yellow stained cell appeared; positive expression (+), positive cell count of each high power field < 20%; dominant expression (++), positive cell count of each high power field was in the range from 20% to 50%; strong positive expression (+++), positive cell count of each high power field was > 50%. Ridit analysis statistical method was used for the correlation analysis. MAIN OUTCOME MEASURES: Expression of HSP70 and c-fos genes. RESULTS: Totally 148 cases of primary hypertension participated in the experiment and the blood sample of all of those entered the result analysis. ① Six cases presented positive expression of both HSP70 and c-fos genes. ② Four cases presented dominant expression of HSP70 gene and positive expression of c-fos gene respectively. ③ Twelve cases presented strong positive expression of HSP70 gene and positive expression of c-fos gene respectively. ④ Six cases presented positive expression of HSP70 gene and dominant expression of c-fos gene respectively. ⑤ Nineteen cases presented dominant expression of both HSP70 and c-fos genes. ⑥ Sixteen cases presented strong positive expression of HSP70 gene and dominant expression of c-fos gene respectively. ⑦ One case presented positive expression of HSP70 gene and strong positive expression of c-fos gene respectively. ⑧ Twenty-eight cases presented dominant expression of HSP70 gene and strong positive expression of c-fos respectively. ⑨ Fifty-six cases presented strong positive expression of both HSP70 and c-fos genes. ⑩ The grade of positive expression presented significant correlation between HSP70 gene and c-fos gene (R =0.281 1-0.644 6, P < 0.01). CONCLUSION: In blood stasis syndrome of primary hypertension, the high expression presents in both HSP70 and c-fos genes, indicating remarkably correlation.