烧伤厌氧菌快速检测的实验研究及临床应用

Experimental Study and Clinical Application of Rapid Diagnosis of Anaerobic Infection

为探索厌氧菌快速检测方法，采用厌氧菌培养法、间接荧光法、酶标染色法和气相色谱法，对烫伤注菌大鼠１２０份标本中脆弱类杆菌与产气荚膜杆菌进行了对比检测。在对１１０份外科标本作厌氧培养的同时，对产黑类杆菌、脆弱类杆菌、产气荚膜杆菌、核梭杆菌和消化链球菌用荧光法和酶染法进行了对比，制备了后四种菌的混合抗体，并与其单一抗体染色结果进行了比较研究。结果表明：（１）荧光法和酶染法检出厌氧菌快速简便、敏感特异；（２）对形态不同的厌氧菌可制成混合抗体进行检测，以扩大检测范围；（３）色谱法在具备条件的情况下也可快速检出厌氧菌。

To explore a rapid diagnosis of anaerobic infection, culture method (CM), indirect immunofluorescent antibody technique (IFA), enzyme labelled antibody technique (ELAT) and gas chromatography (GC) were used and 120 animal specimens and 110 clinical specimens were collected.It was found that the positive rate was higher in IFA and ELAT than in CM and GC while there was no significant difference between IFA and ELAT or between CM and GC. Mixed antibodies of four common encountered anaerobes with different morphology were prepared to extend the detectable range. The detectable rate made no difference with the individual antibody.These results suggest that IFA and ELAT are rapid, simple, specific and sensitive methods for the diagnosis of anaerobic infection; GC may be used under certain conditions, and compound antibodies could be used to extend the detectable species. (Original article on page 371)