念珠菌检测与药敏分析

Candida Detection and Analysis of Drug Sensitivity

目的探讨念珠菌检出率及其药敏特点,为临床感染性疾病提供病原学诊断和合理运用抗真菌药物的依据。方法采集6660份各种临床标本,接种于真菌平板及血平板,疑为酵母样真菌者采用YBC卡进一步鉴定,并对所有分离出的念珠菌做抗真菌药敏实验。结果共检出313株念珠菌,其中白色念珠菌(Cal)248株、热带念珠菌(Ctr)35株、近光滑念珠菌(Cpa)17株、季也蒙念珠菌(Cgu)9株、腐生念珠菌(Chu)2株、克柔念珠菌(Ckr)和星状念珠菌(Clm)各1株,对5氟胞嘧啶(5Fc)、克霉唑(CTR)、两性霉素(AMB)、咪康唑(MCZ)、酮康唑(KET)、益康唑(ECO)、制霉菌素(NTS)和氟康唑(FLC)的平均敏感性分别是:Cal85.6%,Ctr80.9%,Cpa89.5%,Cgu68.9%,Chu93.8%,Ckr12.5%和Clm37.5%。结论目前临床送检念珠菌的标本以尿液、前列腺液、阴道分泌物和痰液为主,而咽拭子、痰液和阴道分泌物检出率较高,分离出的念珠菌以Cal为主,各种念珠菌对临床上常用抗真菌药物的敏感性具有明显不同。

Objective To study the positive rate of Candidas and their characteristics of drug sensitivity,and provide guideline of etiologic diagnosis and suitable anti-fungus medicine for clinical infectious diseases.Methods A total of 6660 samples was collected and inoculated into fungi disk and blood disk.The suspect yeast-like fungi were farther identified with YBC cards and tested for drug sensitive.Results 313 strains of Candidasis were identified,including 248 Candida albicans,35 Candida tropicalis,17 Candida parapsilosis,9 Candida guiliermondii,2 Candida humicola,1 Candida krusei and 1 Candida lumbica.Their average drug sensitive ratios to 5-Fc,CTR,AMB,MCZ,KET,ECO,NTS and 5-Fc were 85.6%,80.9%,89.5%,68.9%,93.8%,12.5% and 37.5% respectively.Conclusion The main samples for Candidasis detecting were urine,prostate liquid,vaginal secretions and sputums.But the positive rate of Candida detecting in vaginal secretions,sputums and throat swaps was higher and the major candida is Candida albicans.Every Candida was different on anti- fungusm medicine.