期刊文献+
任意字段
题名或关键词
题名
关键词
文摘
作者
第一作者
机构
刊名
分类号
参考文献
作者简介
基金资助
栏目信息

免疫荧光法观察正畸大鼠牙髓CGRP的变化 被引量：2

Expression of calcitonin gene-related peptide in rat dental pulp during tooth movement
下载PDF
导出
摘要 目的:观察正畸牙齿移动不同时期大鼠牙髓组织中降钙素基因相关肽(CGRP)阳性神经纤维的变化。方法:采用冷冻切片和间接免疫荧光方法观察正畸大鼠牙髓组织CGRP阳性神经纤维的表达变化情况。结果:CGRP阳性神经纤维在大鼠牙髓组织中分布较丰富,在根髓呈粗大的束状;在冠髓呈条索状、串珠样,并在髓顶造牙本质细胞下层,成网状分布。正畸加力后3d大鼠牙髓CGRP阳性神经纤维数量开始增加;加力7d达到最高;至撤力后28d下降到正常水平。结论:CGRP阳性神经纤维参与了正畸牙齿移动大鼠牙髓组织的反应过程。 Objectives:To investigate the relationship between calcitonin gene-related peptide(CGRP) in rat dental pulp and tooth movement.Methods:The first maxillary molars in 36 SD rats were moved mesially by orthodontic force,18 were examined 3,7 and 14 day after application of the appliance whereas another 18 rats were observed 7,14 and 28 days after removal of the appliance respectively.6 control rats were without treatment.Then,CGRP immunoreactivity was demonstrated by indirect immunoflurescence on frozen sections of dental pulp samples.Results:3,7 and 14 days after application of orthodontic force, the CGRP containing nurve fiber counts in each pulp were 17.57±4.42,24.04±3.55 and 21±4.11 respectively,those in the control pulps were 8.03±4.49. 7,14 and 28 days after removal of the appliance, the counts were 19.23±5.23,18.23±5.08 and 8.12±5.01 respectively.Conclusions:CGRP immunoreactive nerve fibres may take an active part in tissue responses in pulp tissues during experiment tooth movement.
作者 孙应明 罗颂椒 赵昱辉
机构地区 四川大学华西口腔医学院正畸科 第四军医大学口腔医学院正畸科
出处 《实用口腔医学杂志》 CAS CSCD 北大核心 2005年第4期474-476,共3页 Journal of Practical Stomatology
基金 国家自然基金资助项目(30200320)
关键词 降钙素基因相关肽 牙髓 牙齿移动 免疫荧光 Calcitonin gene-related peptide Dental pulp Tooth movement Immunoflurescence
分类号 R783.5 [医药卫生—口腔医学]
  • 相关文献

参考文献8

  • 1Amara SG, Jonas V, Rosenfeld MG, et al. Alternative RNA processing in calcitonin gene expression generates mRNAs enconding defferent polypeptide products. Nature, 1982, 298(5871) :240
  • 2Kerezoudis NP, Olgart L, Edwall L. CGRP(8-37) reduces the duration but not the maximal increase of antidromic vasolidation in dental pulp and lip of the rat. Acta Physiol Scand,1994,151 ( 1 ) :73
  • 3Yamaguchi M, Kojima T, Kanekawa M, et al. Neuropeptides stimulate production of interleukin-1 beta, interleukin-6, and tumor necrosis factor-alpha in human dental pulp cells. Inflamm Res,2004,53 (5): 199
  • 4陆群,吴补领,张洪伟,苏凌云,余擎.降钙素基因相关肽对人牙髓细胞的矿化影响[J].实用口腔医学杂志,2002,18(1):36-38. 被引量：6
  • 5Vandevska-Radunovic V, Kvinnsland IH,et al. SEffect of inferior alveolar nerve axotomy on periodontal and pulpal blood flow subsequent to experimental tooth movement in rats. Acta Odontol Scand, 1998,56( 1 ) :57
  • 6Kvinnsland I, Kvinnsland S. Changes in CGRP-immunoreactive nerve fibres during experimental tooth movement in rats.Eur J Orthod, 1990,12 (3) :320
  • 7Vandevska-Radunovic V, Kvinnsland IH, Kvinnsland S.Effect of experimental tooth movement on nerve fibres immunoreactive to calcitonin gene-related peptide, protein gene product 9.5, and blood vessel density and distribution in rats. Eur J Orthod, 1997,19 (5) :517
  • 8Norevall LI, Forsgren S, Matsson L. Expression of neuropeptides (CGRP, substance P ) during and after orthodontic tooth movement in the rat. Eur J Orthod, 1995,17 (4) :311

二级参考文献7

  • 1[1]Brain SD,Williams TJ,Tippins JP, et al. Calcitonin gene related peptide is a potent vasodialator. Nature, 1985,313 :54
  • 2[2]Byers MR, Taylor PE. Effect of sensory denervation on the response of rat molar pulp to exposure injury. J Dent Res,1993,72:613
  • 3[3]Tayor PE, Byers MR, Redd PE. Sprouting of CGRP nerve fibers in response to dentin injury in rat molars. Brain Res,1988,461:371
  • 4[4]Bemard GW, Shih C. The osteogenic stimulating effect of neuroactive calcitonin gene related peptide. Peptides, 1990,11:625
  • 5[5]Zhang L, Leeman E, Cames DL, et al. Human osteoblast synthesize and respond to platelet derived growth factor. Am J Physiol, 1991,261:348
  • 6[6]Poyner DR. Calcitonin gene related peptide: Multiple actions, multiple receptors. Pharmac Ther, 1992,56:23
  • 7[7]Calland JW, Harris SE, Carnes DL. Hurnan pulp cells respond to calcitonin gene-related peptide in vitro. Endodontics,1997,23:485

共引文献5

同被引文献52

  • 1赵昱辉,段银钟,孙应明.CGRP1受体在大鼠牙齿及牙周组织中的表达[J].临床口腔医学杂志,2004,20(11):643-645. 被引量：3
  • 2赵姝哲,孙新华,陈思韩.弱激光照射对正畸施力初牙髓组织P物质表达的影响[J].北京口腔医学,2007,15(3):139-140. 被引量：6
  • 3y, Grunheid T, Morbach BA, Zentner A. Pulpal cellular re- actions to experimental tooth movement in rats[J]. Oral Surg Oral Med Oral Pathol Oral Radiol Endod, 2007, 104 (3) : 434-441.
  • 4Santamaria M Jr, Milagres D, Iyomasa MM, et al. Initial pulp changes during orthodontic movement: Histomor- phological evaluatin[J]. Braz Dent J, 2007, t8(1):34-39.
  • 5Shigehara S, Matsuzaka K, Inoue T. Morphohistological change and expression of HSPT0, osteopontin and osteo- calcin mRNAs in rat dental pulp cells with orthodontic tooth movement[J]. Bull Tokyo Dent Coil, 2006, 47 (3): 117-124.
  • 6Konno Y, Daimaruya T, Likubo M, et al. Morphologic and hemodynamic analysis of dental pulp in dogs after molar intrusion with the skeletal anchorage system[J]. Am J Orthod Dentofacial Orthop, 2007, 132 (2): 199- 207. i.
  • 7Tripuwabhrut P,i!Brudvik P, Fristad I, et al. Experimen- tal orthodontic tooth movement and extensive root re- sorption: Periodontal and pulpal changes[J]. Eur J Oral Sci, 2010, 118 (6) : 596-603.
  • 8McDonald F, Pitt Ford TR. Blood flow changes in per- manent maxillary canines during retraction[J]. Eur J Or- thod, 1994, 16(1):1-9.
  • 9Santamaria M 3, Milagres D, Stuani AS, et al. Initial changes in pulpal microvasculature during orthodontic tooth movementi', A stereological study[J]. Eur J Orthod,2006, 28 (3) .. 217-220.
  • 10Ikawa M, Fujiwara M, Horiuchi H, et al. The effect of short-term tooth intrusion on. human pulpal blood flow measured by laser Doppler flowmetry[J]. Arch Oral Biol, 2001, 46(9) :781-787.

引证文献2

二级引证文献4

实用口腔医学杂志
2005年 第4期

相关作者

内容加载中请稍等...

相关机构

内容加载中请稍等...

相关主题

内容加载中请稍等...

浏览历史

内容加载中请稍等...
;
使用帮助 返回顶部